Anti-KIF5A抗体(ab5628)
Key features and details
- Rabbit polyclonal to KIF5A
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-KIF5A抗体
参阅全部 KIF5A 一抗 -
描述
兔多克隆抗体to KIF5A -
宿主
Rabbit -
特异性
This antibody is specific for KIF5A and does not detect other kinesin isotypes.
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经测试应用
适用于: ICC/IF, WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.
Sequence:CGYEAEDQAKLFPLHQETAAS
(Peptide available asab41781) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa.
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IP | (1) |
Use at an assay dependent concentration. PubMed: 20094756
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说明 |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa. |
IP
Use at an assay dependent concentration. PubMed: 20094756 |
靶标
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功能
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). -
组织特异性
Distributed throughout the CNS but is highly enriched in subsets of neurons. -
疾病相关
Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. -
序列相似性
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
结构域
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
细胞定位
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 511273 Cow
- Entrez Gene: 3798 Human
- Entrez Gene: 16572 Mouse
- Entrez Gene: 314906 Rat
- Omim: 602821 Human
- SwissProt: Q12840 Human
- SwissProt: P33175 Mouse
- SwissProt: Q6QLM7 Rat
see all -
别名
- D12S1889 antibody
- KIF 5A antibody
- Kif5a antibody
see all
图片
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Immunofluorescence analysis of KIF5A in SH-SY5Y cells using ab5628. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton™ X-100, and blocked with 5% BSA. The cells were labeled with ab5628 at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.
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All lanes : Anti-KIF5A antibody (ab5628) at 2 µg/ml
Lane 1 : U-87 MG whole cell extracts
Lane 2 : HT-29 whole cell extracts
Lane 3 : C2C12 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 117 kDaBlocking with 5 % skimmed milk
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ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab5628 被引用在 21 文献中.
- Yokota S et al. Kif5a Regulates Mitochondrial Transport in Developing Retinal Ganglion Cells In Vitro. Invest Ophthalmol Vis Sci 64:4 (2023). PubMed: 36862119
- Shah SH et al. Quantitative transportomics identifies Kif5a as a major regulator of neurodegeneration. Elife 11:N/A (2022). PubMed: 35259089
- Pant DC et al. ALS-linked KIF5A ?Exon27 mutant causes neuronal toxicity through gain-of-function. EMBO Rep 23:e54234 (2022). PubMed: 35735139
- Wang XS et al. Mesenchymal stem cells shuttling miR-503 via extracellular vesicles enhance glioma immune escape. Oncoimmunology 11:1965317 (2022). PubMed: 36524211
- Mou Y et al. MFN2 Deficiency Impairs Mitochondrial Transport and Downregulates Motor Protein Expression in Human Spinal Motor Neurons. Front Mol Neurosci 14:727552 (2021). PubMed: 34602978