Anti-KIF4A/KIF4抗体(ab72412)
Key features and details
- Rabbit polyclonal to KIF4A/KIF4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIF4A/KIF4抗体
参阅全部 KIF4A/KIF4 一抗 -
描述
兔多克隆抗体to KIF4A/KIF4 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Pig, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human KIF4A/KIF4 (C terminal).
Database link: NP_036442.3 -
阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72412 was affinity purified using an epitope specific to KIF4A/KIF4 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72412于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
组织特异性
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
序列相似性
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
细胞定位
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 24137 Human
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- Unigene: 648326 Human
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别名
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
图片
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Western blot - Anti-KIF4A/KIF4 antibody (ab72412)All lanes : Anti-KIF4A/KIF4 antibody (ab72412) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 140 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-KIF4A/KIF4 antibody (ab72412)Detection of KIF4A/KIF4 by Western Blot of Immunprecipitate.
ab72412 at 1µg/ml staining KIF4A/KIF4 in HeLa whole cell lysate immunoprecipitated using ab72412 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72412 尚未被引用在任何文献中。