Anti-KIF4A/KIF4抗体(ab3815)

Thank you for taking the time tocontact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from this second vial of antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time the customer has spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there would be no further tips to provide on this occasion to help improve the results andI apologize for the inconvenience. I'm pleased to offer you acredit note or alternative replacementin compensation, which may be more appropriate in this case than another vial of the same antibody.

In addition, I would appreciate if you could confirm the following information which will help with our quality monitoring:

1. Please confirm if the same type of samples have been used in this case (HeLa cells).

2. Has the same procedure (with the suggestions previously provided) been used?

3. What is the problem with the results? Is there high background in WB and ICC again?

4. Has the customer tried fresh samples?

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab3815 has been added to your order number [BO] 88841. This has Abcam order reference 1112739.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

Thank you for your reply.

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggestthe customer has received a bad vial on this occasion.

Therefore, we would be pleased to provide a replacement from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand the customers concerns and if they prefer a credit note in this case, I will be pleased to arrange this for them.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

Thank you for your message and for kindlyproviding this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

I appreciate the time you have spent on these experiments, and would like to offer a refund or credit note in compensation (providing the product has been purchased in the last 6 months). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase as previously requested?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab3815 is tested and covered by our 6 month guarantee for use in WB, ICC-IF and inhuman samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimise the results. I would also appreciate if youare able to confirm some further details:

ICC-IF

1. Has the quality of the sample been assessed, for example what was the% confluency before staining, and was a spare sample checked for viability using trypan blue count before staining?

2. Fixation: To prevent over fixation, whichever method is used, I can suggest this should be for 10 minutes only if this has not already been tried. PFA should be 4% only.

3. Please provide further information regarding the permeabilization. I would suggest to try 0.2% triton, 10 minutes only.

4. I can recommend to consider reducing the concentration to 1:500 to reduce the background signal.

5. I would appreciate if you are able to provide an image which would help us to assess the results.

WB

1. I can suggest to try reducingthe concentration to 1:500 to reduce the background signal. Incubate overnight 4oC.

2. Could you confirm if the quality of sample been assessed using a loading control?

3. Have the samples reduced and denatured?

4. I would appreciate if you are able to provide an image which would help us to assess the results.

General:

1. Please confirm the order number and date of purchase.

2. Is the current vial of secondary antibody working well with other primary antibodies?

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.