Anti-KIF4A/KIF4抗体(ab3815)
Key features and details
- Goat polyclonal to KIF4A/KIF4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KIF4A/KIF4抗体
参阅全部 KIF4A/KIF4 一抗 -
描述
山羊多克隆抗体to KIF4A/KIF4 -
宿主
Goat -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human KIF4A/KIF4 aa 1219-1232 (C terminal).
Sequence:SFFSGCSPIEEEAH
(Peptide available asab22955) -
阳性对照
- WB: NIH/3T3 nuclear lysate. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
靶标
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功能
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
组织特异性
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
序列相似性
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
细胞定位
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 24137 Human
- Entrez Gene: 16571 Mouse
- Entrez Gene: 84393 Rat
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- SwissProt: P33174 Mouse
- Unigene: 648326 Human
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别名
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
图片
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Western blot - Anti-KIF4A/KIF4 antibody (ab3815)All lanes : Anti-KIF4A/KIF4 antibody (ab3815) at 2 µg/ml
Lane 1 : NIH/3T3 nuclear lysate
Lane 2 : Human ovary lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 140 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (ab3815)Immunocytochemical analysis of paraformaldehyde-fixed U-2 OS cells permeabilized with 0.15% Triton. Cells were incubated with ab3815 at 10 ug/ml for 1 hour followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green). The nuclear stain is DAPI (blue). Negative control is unimmunized goat IgG at 10 ug/ml, followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab3815 被引用在 6 文献中.
- Champion L et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). PubMed: 30586323
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201
- Hu CK et al. Midbody assembly and its regulation during cytokinesis. Mol Biol Cell 23:1024-34 (2012). ICC/IF . PubMed: 22278743
- Hu CK et al. Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell 23:2702-11 (2012). ICC/IF ; Human . PubMed: 22621898
- Hu CK et al. KIF4 regulates midzone length during cytokinesis. Curr Biol 21:815-24 (2011). PubMed: 21565503
- Wilmeth LJ et al. Mutual dependence of Mob1 and the chromosomal passenger complex for localization during mitosis. Mol Biol Cell 21:380-92 (2010). Human . PubMed: 19955215