Anti-KIF4A/KIF4抗体(ab122227)
Key features and details
- Rabbit polyclonal to KIF4A/KIF4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIF4A/KIF4抗体
参阅全部 KIF4A/KIF4 一抗 -
描述
兔多克隆抗体to KIF4A/KIF4 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human KIF4A/KIF4 aa 994-1075.
Sequence:LTLLQVASRQKHLPKDTLLSPDSSFEYVPPKPKPSRVKEKFLEQSMDIED LKYCSEHSVNEHEDGDGDDDEGDDEEWKPTKL
Database link: O95239 -
阳性对照
- WB: RT-4 and U-251 MG cell lysates IHC-P: Human bone marrow tissue ICC/IF: Human cell line U-2 OS
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
组织特异性
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
序列相似性
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
细胞定位
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 24137 Human
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- Unigene: 648326 Human
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别名
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
图片
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Western blot - Anti-KIF4A/KIF4 antibody (ab122227)All lanes : Anti-KIF4A/KIF4 antibody (ab122227) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (ab122227)ab122227, at a 1/300 dilution, staining KIF4A/KIF4 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (ab122227)Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab122227.
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (ab122227)Immunohistochemical staining of Human bone marrow shows strong nuclear positivity in hematopoietic cells using ab122227 -
Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (ab122227)ab122227, at 4 µg/ml, staining KIF4A/KIF4 in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab122227 被引用在 11 文献中.
- Zhang C et al. Knockdown of kinesin family member 4A inhibits cell proliferation, migration, and invasion while promoting apoptosis of urothelial bladder carcinoma cells. Cancer Med 12:12581-12592 (2023). PubMed: 37039264
- Zheng P et al. KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3. Int J Mol Med 47:N/A (2021). PubMed: 33846765
- Pan J et al. Identification of KIF4A as a pan-cancer diagnostic and prognostic biomarker via bioinformatics analysis and validation in osteosarcoma cell lines. PeerJ 9:e11455 (2021). PubMed: 34055488
- Wang L et al. Identification of KIF4A as a prognostic biomarker for esophageal squamous cell carcinoma. Aging (Albany NY) 13:24050-24070 (2021). PubMed: 34775374
- Chen J et al. KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion. Biomed Res Int 2021:8249293 (2021). PubMed: 34805404