AB108535

Anti-KIF22抗体[EP2748]

Anti-KIF22 antibody [EP2748]

RabMAb
KO Validated
了解详情

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(1 Publication)

Rabbit Monoclonal KIF22 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

KID, KNSL4, KIF22, Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4

2 Images

Lab

Western blot - Anti-KIF22 antibody [EP2748] (AB108535)

Lanes 1 - 2 : Merged signal (red and green). Green - ab108535 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108535 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KIF22 antibody [EP2748] (ab108535) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

KIF22 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human KIF22 knockout HEK-293T cell line (<a href='/products/cell-lines/human-kif22-knockout-hek-293t-cell-line-ab266817'>ab266817</a>)

Predicted band size: 107 kDa,130 kDa,22 kDa,33 kDa,34 kDa,38 kDa,42 kDa,43 kDa,60 kDa,73 kDa,96 kDa

Observed band size: 107 kDa,17 kDa,33 kDa,34 kDa,42 kDa,70 kDa,75 kDa

false

Unknown

Western blot - Anti-KIF22 antibody [EP2748] (AB108535)

All lanes:

Western blot - Anti-KIF22 antibody [EP2748] (ab108535) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-KIF22 antibody [EP2748] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2748

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed : 25743205).

See full target information KIF22

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:666361 PubMed34168643

2021

M2a Macrophage-Secreted CHI3L1 Promotes Extracellular Matrix Metabolic Imbalances Activation of IL-13Rα2/MAPK Pathway in Rat Intervertebral Disc Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Long Li,Kang Wei,Yifan Ding,Paerxiati Ahati,Haoran Xu,Huang Fang,Huan Wang

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIF22抗体[EP2748]