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使用敲除细胞株进行验证重组RabMAb

重组Anti-KIF22抗体[EP2747Y] (ab75783)

Reviews (1)Q&A (1)References (3)
Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)
  • Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2747Y] to KIF22
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-KIF22抗体[EP2747Y]
    参阅全部 KIF22 一抗

  • 描述

    兔单克隆抗体[EP2747Y] to KIF22

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, 283T, and Wild-type HEK-293T cell lysates.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 31st July 2023.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP2747Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75783于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/5000. Predicted molecular weight: 73 kDa.
说明
WB
1/5000. Predicted molecular weight: 73 kDa.

靶标

  • 功能

    Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.

  • 序列相似性

    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.

  • 翻译后修饰

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 数据库链接

    • 别名

      • A 328A3.2 antibody
      • KID antibody
      • KIF 22 antibody
      • kif22 antibody
      • KIF22_HUMAN antibody
      • Kinesin family member 22 antibody
      • Kinesin like 4 antibody
      • Kinesin like DNA binding protein antibody
      • Kinesin like DNA binding protein pseudogene antibody
      • Kinesin like protein 4 antibody
      • Kinesin like protein KIF22 antibody
      • Kinesin-like DNA-binding protein antibody
      • Kinesin-like protein 4 antibody
      • Kinesin-like protein KIF22 antibody
      • KNSL 4 antibody
      • KNSL4 antibody
      • OBP 1 antibody
      • OBP 2 antibody
      • OBP antibody
      • OBP1 antibody
      • OBP2 antibody
      • Origin of plasmid DNA replication binding protein antibody
      • OriP binding protein antibody
      • OTTHUMP00000123406 antibody
      • SEMDJL2 antibody
      see all

    图片

    • Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)
      Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)
      All lanes : Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method
      Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size: 73 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 40 seconds
    • Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)
      Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)
      All lanes : Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : KIF22 knockout HEK-293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 73 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      This image was generated using the hybridoma version of this product.

      Lanes 1 - 2: Merged signal (red and green). Green - ab75783 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab75783 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75783 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    文献 (3)

    发表研究结果有使用 ab75783？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab75783 被引用在 3 文献中.

    • Ren Y  et al. GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling. Cell Death Discov 8:208 (2022). PubMed: 35436989
    • Schneider MWG  et al. A mitotic chromatin phase transition prevents perforation by microtubules. Nature 609:183-190 (2022). PubMed: 35922507
    • Li C  et al. NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nat Commun 7:10597 (2016). IF . PubMed: 26839278

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-KIF22 antibody [EP2747Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (cartilage)
    Loading amount
    10 µg
    Specification
    cartilage
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 26 2012

    Question

    Hi, I am interested in a KIF22/KID antibody that will work in zebrafish. None of your antibodies have zebrafish cross reactivity information. Where an epitope for the immunogen has been specified I've checked and the human vs zebrafish sequence varies quite a bit. Can you tell me which of the following antibodies has the best chance of working? Please let me know if you need more info to answer my question. Zebrafish accession # NP_001107063 Antibody codes: ab56235 ab66453 ab72261 ab69824 ab75783 ab108535 Thanks,

    Read More
    Answer

    Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. I have checked the alignment of the immunogen sequences for the available antibodies with the zebrafish sequence. I am sorry that the alignment is very low in all cases, and regrettably we would not predict that any of the KIF22 antibodies would detect in Zebrafish. I am sorry that this is not better news for you on this occasion, and that we have no suitably tested KIFF22 antibodies available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions.          

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