AB75783

重组Anti-KIF22抗体[EP2747Y]

Name: KIF22抗体[EP2747Y] (ab75783)| Abcam中文官网
Brand: Abcam Limited
SKU: AB75783
Price: 1668 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-KIF22 antibody [EP2747Y]

RabMAb
KO Validated
20ul selling size
了解详情

(1 Review)

(4 Publications)

Anti-KIF22 antibody [EP2747Y] (ab75783) is a rabbit monoclonal antibody detecting KIF22 in Western Blot. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2009

查看别名

KID, KNSL4, KIF22, Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4

2 Images

Supplier Data

Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)

All lanes:

Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

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Exposure time: 40s

Lab

Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)

This image was generated using the hybridoma version of this product.

Lanes 1 - 2 : Merged signal (red and green). Green - ab75783 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab75783 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab75783 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

KIF22 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human KIF22 knockout HEK-293T cell line (<a href='/products/cell-lines/human-kif22-knockout-hek-293t-cell-line-ab266817'>ab266817</a>)

Predicted band size: 73 kDa

Observed band size: 75 kDa

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不同偶联物与剂型 (1)

Carrier free

Anti-KIF22 antibody [EP2747Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2747Y

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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产品详情

What is this antibody validated in?
Anti-KIF22 antibody [EP2747Y] (ab75783) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of KIF22?
Anti-KIF22 [EP2747Y] (ab75783) specifically detects a band for KIF22 (UniProt: Q14807) at a molecular weight of 73kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-KIF22 antibody [EP2747Y] (ab75783) has been confirmed by Western blot testing in KIF22 Knockout HEK-293 cells.

Other related products
We have a range of other formats of antibody clone [EP2747Y] also available for your convenience: ab75783, Carrier free - ab284718

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed : 25743205).

See full target information KIF22

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003086 PubMed40208872

2025

A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Iratxe Zuazo-Gaztelu,David Lawrence,Ioanna Oikonomidi,Scot Marsters,Ximo Pechuan-Jorge,Catarina J Gaspar,David Kan,Ehud Segal,Kevin Clark,Maureen Beresini,Marie-Gabrielle Braun,Joachim Rudolph,Zora Modrusan,Meena Choi,Wendy Sandoval,Mike Reichelt,David C DeWitt,Pekka Kujala,Suzanne van Dijk,Judith Klumperman,Avi Ashkenazi

Nature 609:183-190 PubMed35922507

2022

A mitotic chromatin phase transition prevents perforation by microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian W G Schneider,Bryan A Gibson,Shotaro Otsuka,Maximilian F D Spicer,Mina Petrovic,Claudia Blaukopf,Christoph C H Langer,Paul Batty,Thejaswi Nagaraju,Lynda K Doolittle,Michael K Rosen,Daniel W Gerlich

Cell death discovery 8:208 PubMed35436989

2022

GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yeqing Ren,Biao Yang,Geng Guo,Jianping Zhang,Yanqi Sun,Dong Liu,Shihao Guo,Yongqiang Wu,Xiaogang Wang,Shule Wang,Wenju Zhang,Xiaolong Guo,Xuepeng Li,Ren Li,Jianhang He,Zihan Zhou

Nature communications 7:10597 PubMed26839278

2016

NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force.

Applications

Species

Unspecified reactive species

Chenyu Li,Chenyi Xue,Qiaoyun Yang,Boon Chuan Low,Yih-Cherng Liou

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-KIF22抗体[EP2747Y]