Anti-KIF18B抗体(ab121798)
Key features and details
- Rabbit polyclonal to KIF18B
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIF18B抗体
参阅全部 KIF18B 一抗 -
描述
兔多克隆抗体to KIF18B -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 15-111 (STLQVVVRVR PPTPRELDSQ RRPVVQVVDE RVLVFNPEEP DGGFPGLKWG GTHDGPKKKG KDLTFVFDRV FGEAATQQDV FQHTTHSVLD SFLQGYN) of Human KIF18B.
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阳性对照
- IHC-P: Human lymph node and duodenum tissues; ICC: Human U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121798于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
In complex with KIF2C, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Its major role may be to transport KIF2C and/or MAPRE1 along microtubules. -
组织特异性
Shows a prominent expression in the amygdala. -
序列相似性
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Contains 1 kinesin motor domain. -
发展阶段
Regulated in a cell cycle-dependent manner. Not expressed in the G1 phase. In G2, sequestered in the nucleus. Maximal levels seen at late G2/M phase and early prometaphase. Degraded at the metaphase-anaphase transition (at protein level). -
细胞定位
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Present predominantly in the nucleus and to a lesser extent in the cytoplasm of interphase cells. During mitosis, found to be closely associated with astral microtubule plus ends emanating from the spindle pole during prometaphase and metaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 146909 Human
- Omim: 614570 Human
- SwissProt: Q86Y91 Human
- Unigene: 135094 Human
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别名
- KI18B_HUMAN antibody
- KIF18B antibody
- Kinesin family member 18B antibody
- Kinesin-like protein KIF18B antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18B antibody (ab121798)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling KIF18B with ab121798 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-KIF18B antibody (ab121798)Immunocytochemistry analysis of U-251 MG (human brain glioma cell line) cells labelling KIF18B with ab121798 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18B antibody (ab121798)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling KIF18B with ab121798 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18B antibody (ab121798)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling KIF18B with ab121798 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18B antibody (ab121798)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling KIF18B with ab121798 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab121798 被引用在 6 文献中.
- Zhu Y et al. Identification of potential circular RNA biomarkers in lung adenocarcinoma: A bioinformatics analysis and retrospective clinical study. Oncol Lett 23:144 (2022). PubMed: 35340554
- Li B et al. KIF18B promotes the proliferation of pancreatic ductal adenocarcinoma via activating the expression of CDCA8. J Cell Physiol 235:4227-4238 (2020). PubMed: 31875977
- Gao T et al. KIF18B promotes tumor progression in osteosarcoma by activating ß-catenin. Cancer Biol Med 17:371-386 (2020). PubMed: 32587775
- Liu W et al. Silencing KIF18B enhances radiosensitivity: identification of a promising therapeutic target in sarcoma. EBioMedicine 61:103056 (2020). PubMed: 33038765
- Zhao F et al. Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma. Cancer Manag Res 12:12769-12778 (2020). PubMed: 33335427
- Andersson E et al. Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets. Mod Pathol 29:616-29 (2016). PubMed: 26965582