Anti-KIF14抗体(ab3746)
Key features and details
- Rabbit polyclonal to KIF14
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIF14抗体
参阅全部 KIF14 一抗 -
描述
兔多克隆抗体to KIF14 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human KIF14 aa 50-150.
Database link: Q15058 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IP |
Use at 1-4 µg/mg of lysate.
|
说明 |
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IP
Use at 1-4 µg/mg of lysate. |
靶标
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功能
Plays an essential role in cytokinesis. -
序列相似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
发展阶段
Up-regulated in cells progressing through G2/M phase. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring. - Information by UniProt
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数据库链接
- Entrez Gene: 9928 Human
- Omim: 611279 Human
- SwissProt: Q15058 Human
- Unigene: 3104 Human
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别名
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
see all
图片
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KIF14 was immunoprecipitated from 50 µg/ml and 1 mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab3746 at 3 µg/ml. Western blot was performed from the immunoprecipitate using ab3746 at 1 µg/ml.
Lane 1: HeLa whole cell lysate 50 µg (Input).
Lane 2: ab3746 IP in HeLa whole cell lysate.
Lane 3: Ctrl IgG instead of ab3746.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab3746 被引用在 6 文献中.
- Vukušic K et al. Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Dev Cell 56:1253-1267.e10 (2021). PubMed: 33910056
- Malaby HLH et al. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol 218:1218-1234 (2019). PubMed: 30709852
- Reilly ML et al. Loss-of-function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish. Hum Mol Genet 28:778-795 (2019). PubMed: 30388224
- Wang ZZ et al. KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer. Int J Oncol 53:1939-1952 (2018). PubMed: 30226594
- Sgrò F et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). PubMed: 26586574
- Carleton M et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF ; Human . PubMed: 16648480