Anti-KIAA0652/ATG13抗体(ab105392)
Key features and details
- Rabbit polyclonal to KIAA0652/ATG13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-KIAA0652/ATG13抗体
参阅全部 KIAA0652/ATG13 一抗 -
描述
兔多克隆抗体to KIAA0652/ATG13 -
宿主
Rabbit -
特异性
ab105392 is predicted to not cross-react with other ATG members. Two isoforms of KIAA0652/ATG13 are known to exist; this antibody will recognize both isoforms.
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经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human KIAA0652/ATG13 (C terminal).
Database link: NP_001136145 -
阳性对照
- WB: Rat heart tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105392于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. -
序列相似性
Belongs to the ATG13 metazoan family. -
翻译后修饰
Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. -
细胞定位
Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. - Information by UniProt
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数据库链接
- Entrez Gene: 9776 Human
- Entrez Gene: 51897 Mouse
- Entrez Gene: 362164 Rat
- SwissProt: O75143 Human
- SwissProt: Q91YI1 Mouse
- Unigene: 127403 Human
- Unigene: 28492 Mouse
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别名
- ATG13 antibody
- ATG13 autophagy related 13 homolog (S. cerevisiae) antibody
- ATG13_HUMAN antibody
see all
图片
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Western blot - Anti-KIAA0652/ATG13 antibody (ab105392)All lanes : Anti-KIAA0652/ATG13 antibody (ab105392) at 1 µg/ml
Lane 1 : Rat heart tissue lysate without blocking peptide
Lane 2 : Rat heart tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 57 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab105392 被引用在 7 文献中.
- Shi Y et al. CircHADHA-augmented autophagy suppresses tumor growth of colon cancer by regulating autophagy-related gene via miR-361. Front Oncol 12:937209 (2022). PubMed: 36313671
- Wang S et al. miR-654-5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Mol Med Rep 23:N/A (2021). PubMed: 33846806
- Stavoe AK et al. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. Elife 8:N/A (2019). PubMed: 31309927
- Zhou M et al. Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway. Front Pharmacol 10:603 (2019). PubMed: 31214032
- Shin JH et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
- Liu W et al. Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway. Int J Mol Med 37:309-18 (2016). WB . PubMed: 26647915
- Huang J et al. HMGB1 promotes drug resistance in osteosarcoma. Cancer Res 72:230-8 (2012). WB ; Human . PubMed: 22102692