Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组

重组Anti-Ki67抗体[BLR021E] - BSA free (ab272075)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
  • Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
  • Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR021E] to Ki67 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Ki67抗体[BLR021E] - BSA free
    参阅全部 Ki67 一抗

  • 描述

    兔单克隆抗体[BLR021E] to Ki67 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Ki67 aa 1200-1250. The exact sequence is proprietary. Corresponds to the repetitive 66 bp element. NP_002408.2 and Gene ID 4288.
    Database link: P46013

  • 阳性对照

    • IHC-P: Human tonsil and ovarian carcinoma tissue. ICC: OVCAR-4 cells. ICC/IF: Acetone/methanol fixed Hela cells.

  • 常规说明

    ab272075 is the carrier-free version of ab243878.

    This product is sold under License from Bethyl Laboratories, Inc.

性能

相关产品

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272075于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

  • 序列相似性

    Contains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.

  • 发展阶段

    Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).

  • 翻译后修饰

    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

  • 细胞定位

    Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Information by UniProt

  • 数据库链接

    • 别名

      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue, labeling Ki67 with ab243878. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Detection: DAB.

    • Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
      Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).

      Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-4 cells, labeling Ki67 with ab243878. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Detection: DAB.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
      Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).

      Immunocytochemical analysis of acetone/menthanol fixed HeLa cells, labeling Ki67 with ab243878 at a 1/100 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Counterstain: DAPI.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue, labeling Ki67 with ab243878 at a 1/500 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Substrate: Opal™. Counterstain: DAPI (blue).

    • Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)
      Anti-Ki67 antibody [BLR021E] - BSA and Azide free (ab272075)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab272075？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272075 被引用在 1 文献中.

    • Chen KL  et al. The protective effect of rosmarinic acid on myotube formation during myoblast differentiation under heat stress. In Vitro Cell Dev Biol Anim 56:635-641 (2020). PubMed: 32901428

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab272075.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com