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Cell Biology Cell Cycle Markers

Anti-Ki67抗体(ab833)

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Reviews (13)Q&A (9)References (168)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)

Key features and details

  • Rabbit polyclonal to Ki67
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Ki67抗体
    参阅全部 Ki67 一抗
  • 描述

    兔多克隆抗体to Ki67
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Ki67. Immunogen is from the 62 base pair region of the human Ki-67 antigen.
    Database link: P46013

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Ki67 peptide (ab15581)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab833于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (7)
1/25 - 1/50.

We recommend using an ABC system for detection of this antibody.

Positive Control: Hu tonsil tissue

说明
IHC-P
1/25 - 1/50.

We recommend using an ABC system for detection of this antibody.

Positive Control: Hu tonsil tissue

靶标

  • 功能

    Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
  • 序列相似性

    Contains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.
  • 发展阶段

    Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
  • 翻译后修饰

    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
  • 细胞定位

    Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Target information above from: UniProt accession P46013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4288 Human
    • Omim: 176741 Human
    • SwissProt: P46013 Human
    • Unigene: 689823 Human
    • Unigene: 80976 Human
    • 别名

      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)This image is courtesy of an abreview submitted by Mithra Mahmoudi.
      HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody ab833 at 5 ug/mL for 30 min at RT.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)Image from Medic S et al., PLoS One. 2010 Apr 22;5(4):e9977. Fig 3.; doi:10.1371/journal.pone.0009977; April 22, 2010, PLoS ONE 5(4): e9977.
      Immunohistochemical analysis of Human skin tissue, staining Ki67 (red) with ab833.

      Antigen retrieval was performed by heat mediation in a EDTA/Tris buffer (pH 8.0). Sections were blocked with 10% NGS for 1 hour at room temperature, followed by incubation with primary antibody (1/25). An AlexFluor®546 anti-rabbit IgG (1/500) was used as the secondary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab833)

      ab833 - immunohistochemistry

      Formalin fixed paraffin embedded human tonsil stained with Ki-67, using ABC and AEC chromogen.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (168)

    发表研究结果有使用 ab833?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab833 被引用在 168 文献中.

    • Maas RGC  et al. Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes. STAR Protoc 2:100334 (2021). PubMed: 33615277
    • Zhang P  et al. Pannexin 3 regulates skin development via Epiprofin. Sci Rep 11:1779 (2021). PubMed: 33469169
    • Diesinger T  et al. A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma. Can J Gastroenterol Hepatol 2021:8854432 (2021). PubMed: 33604316
    • de Miguel-Gómez L  et al. Comparison of different sources of platelet-rich plasma as treatment option for infertility-causing endometrial pathologies. Fertil Steril 115:490-500 (2021). PubMed: 32854930
    • Chen B  et al. CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway. Front Oncol 10:577229 (2020). PubMed: 34079750
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 22 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ki67 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (liver)
    Permeabilization
    Yes - 0.2% Triton x100
    Specification
    liver
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 33% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 17 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    提交于 Sep 25 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    提交于 Aug 20 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (prostate cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.05% trypsin for 10 min at 37°C
    Permeabilization
    No
    Specification
    prostate cancer
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 21 2015

    Immunocytochemistry/ Immunofluorescence

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    African Green Monkey Cell (Cos7 Monkey Kidney Fibroblast)
    Specification
    Cos7 Monkey Kidney Fibroblast
    Permeabilization
    Yes - .3% Triton X100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 13 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Adult skin)
    Specification
    Adult skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 06 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody - Proliferation Marker

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 20 2009

    Immunocytochemistry abreview for Anti-Ki67 antibody - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (retinoblastoma cells)
    Specification
    retinoblastoma cells
    Fixative
    Cytospin solution: methanol, acetone, glycerine
    Permeabilization
    Yes - PBS-Tween
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 14 2009

    Question

    So I have attached an image taken of my staining. As you can notice, the staining is largely nucleolar not perinucleolar. I have indicated cells that have been transfected with white astericks, therefore the non noted cells are non trasnfected. The transfected cells have largely diminished staining, but it is still detectable. Therefore, I am unsure whether these are cells that have become quiescent, or if they are no longer in G1.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2012

    Answer

    >As I mentioned previously, I would recommend running a no primary antibody control to ensure that the diffuse nuclear staining cannot be attributed to non-specific binding. If the no primary antibody control is clear, then I would conclude thatmost of yourtransfected cells are still proliferating.

    Ki67 is not ideal for telling exactly what stage of proliferation your cells are in, butthe first image in this articlemay provide a helpful reference:

    http://www.riken.jp/celldynamics/english/research_3.html

    In quiescent cells, Ki67 should be entirely absent. For example, you could compare your results to Fig 5 a in this paper: http://genesdev.cshlp.org/content/18/1/88/F5.expansion.htmlor Fig 1 F vs G in this paper: http://www.sciencedirect.com/science/article/pii/S1097276507004042

    If you would like to more accurately determine the cell cycle phase of your transfected cells, you could analyze them using propidium iodide (ab14083), DRAQ5 (ab108410),or DRAQ7 (ab109202)in flow cytometry using this protocol:

    https://www.abcam.com/index.html?pageconfig=resource&rid=13432

    Read More

    Abcam Scientific Support

    回复于 Jul 06 2012

    Question

    We are performing immunofluorescence using your anti-ki67 antibody on COS7 cells grown in culture, and see distinct nucleolar staining with a hazy nuclear stain.
    Cells are transfected for 48 hours. Fixed in 4% PFA. Permeabalized in Triton x100 (.5%). Then blocked and stained on coverslips.
    My question lies in how to quantify Ki67 positive and negative cells. Although we see a decrease in nucleolar stain in some of our transfected cells, the cloud of nuclear staining does not disappear. Should we then quantify the amount of of relative fluorescence between the transfected and non transfected cells? Or assume that depletion of Ki67 in the nucleolus indicates a cell in G0? Or is it that all of the stain should disappear for the result to be positive?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 03 2012

    Answer

    Ki67 will be localized in the perinucleolar region in the G1 phase and in the nuclear matrix (your hazy nuclear stain) in the later S, G2, and M phases. During G0, Ki67 will not be detected. If you are looking to quantify interphase / division vs quiescence / senescence, you can simply look for number of cells showing any fluorescence vs the number of cells without Ki67 expression. In order to make sure that the nuclear staining you are observing is specific, it is always recommended to run a no primary antibody control.

    Read More

    Abcam Scientific Support

    回复于 Jul 03 2012

    1-10 of 22 Abreviews or Q&A

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