重组Anti-KGF/FGF-7抗体[EPR7261] (ab131162)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7261] to KGF/FGF-7
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-KGF/FGF-7抗体[EPR7261]
参阅全部 KGF/FGF-7 一抗 -
描述
兔单克隆抗体[EPR7261] to KGF/FGF-7 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Fetal brain and fetal kidney lysates; 293T cell lysate. Human, mouse and rat brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7261 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131162于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. -
组织特异性
Epithelial cell. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2252 Human
- Entrez Gene: 14178 Mouse
- Entrez Gene: 29348 Rat
- Omim: 148180 Human
- SwissProt: P21781 Human
- SwissProt: P36363 Mouse
- SwissProt: Q02195 Rat
- Unigene: 567268 Human
see all -
形式
Potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. -
别名
- FGF 7 antibody
- FGF-7 antibody
- Fgf7 antibody
see all
图片
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All lanes : Anti-KGF/FGF-7 antibody [EPR7261] (ab131162) at 0.1 µg/ml (purified)
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Anti-KGF/FGF-7 antibody [EPR7261] (ab131162) at 0.1 µg/ml (purified) + Human brain lysates at 15 µg
Secondary
Rabbit monoclonal [EPR7261] to KGF/FGF-7 (ab131162) at 1/2000 dilution (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)
Predicted band size: 23 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-KGF/FGF-7 antibody [EPR7261] (ab131162) at 1/1000 dilution (Unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab131162 被引用在 7 文献中.
- Xiang C et al. Circular RNA circLRCH3 promotes oxaliplatin resistance in gastric cancer through the modulation of the miR-383-5p/FGF7 axis. Histol Histopathol N/A:18506 (2022). PubMed: 35920365
- Niimi Y et al. Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds. Sci Rep 11:975 (2021). PubMed: 33441597
- Mi Y et al. Upregulation of Linc-ROR Promotes the Proliferation, Migration, and Invasion of Gastric Cancer Cells Through miR-212-3p/FGF7 Axis. Cancer Manag Res 13:899-912 (2021). PubMed: 33564265
- Feng Y et al. Resveratrol Derivative, Trans-3, 5, 4'-Trimethoxystilbene Sensitizes Osteosarcoma Cells to Apoptosis via ROS-Induced Caspases Activation. Oxid Med Cell Longev 2021:8840692 (2021). PubMed: 33833855
- Yang X et al. Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/ß-catenin. Stem Cell Res Ther 11:182 (2020). PubMed: 32429994
- Zhu Y et al. Long noncoding RNA Linc00460 promotes breast cancer progression by regulating the miR-489-5p/FGF7/AKT axis. Cancer Manag Res 11:5983-6001 (2019). PubMed: 31308741
- Mao Y et al. Repair Effects of KGF on Ischemia-Reperfusion-Induced Flap Injury via Activating Nrf2 Signaling. J Surg Res 244:547-557 (2019). PubMed: 31351398