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重组RabMAb

重组Anti-Keratin 12/K12抗体[EPR1609(2)] (ab124975)

Submit a review Q&A (1)References (6)
Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
  • Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1609(2)] to Keratin 12/K12
  • Suitable for: WB
  • Reacts with: Rat

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Keratin 12/K12抗体[EPR1609(2)]
    参阅全部 Keratin 12/K12 一抗

  • 描述

    兔单克隆抗体[EPR1609(2)] to Keratin 12/K12

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: IP

  • 种属反应性

    与反应: Rat
    预测可用于: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Rat eyeball lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124975于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.

  • 组织特异性

    Cornea specific.

  • 疾病相关

    Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.

  • 序列相似性

    Belongs to the intermediate filament family.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CK-12 antibody
      • Cytokeratin-12 antibody
      • K12 antibody
      • K1C12_HUMAN antibody
      • Keratin 12 antibody
      • Keratin antibody
      • Keratin, type I cytoskeletal 12 antibody
      • Keratin-12 antibody
      • KRT12 antibody
      • type I cytoskeletal 12 antibody
      see all

    图片

    • Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
      Western blot - Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
      Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975) at 1/1000 dilution + Rat eyeball lysate at 10 µg

      Predicted band size: 54 kDa

    • Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)
      Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975)

    文献 (6)

    发表研究结果有使用 ab124975？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124975 被引用在 6 文献中.

    • Li M  et al. Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis. Nat Commun 12:420 (2021). PubMed: 33462242
    • Li M  et al. Loss of FOXC1 contributes to the corneal epithelial fate switch and pathogenesis. Signal Transduct Target Ther 6:5 (2021). PubMed: 33414365
    • Guo D  et al. A Spatiotemporal Requirement for Prickle 1-Mediated PCP Signaling in Eyelid Morphogenesis and Homeostasis. Invest Ophthalmol Vis Sci 59:952-966 (2018). PubMed: 29450535
    • Kitazawa K  et al. PAX6 regulates human corneal epithelium cell identity. Exp Eye Res 154:30-38 (2017). PubMed: 27818314
    • Li G  et al. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. J Biol Chem 290:20448-54 (2015). PubMed: 26045558
    • Ouyang H  et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature 511:358-61 (2014). PubMed: 25030175

    客户评价及客户问答

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    Question

    I need an antibody that is tagged with FITC or APC or some fluorescence so that I can use them in flow cytometry. Im looking for K3 and K12. It would be awesome if you can also provide me with the same information about p63 and ABCG2. Thank you for your time.

    Read More
    Answer

    Thank you for your inquiry and your interest in our products.

    I searched our catalogue and we currently do not have a cytokeratin 3 antibody that is directly conjugated. We do carry ab77869, which has been tested in FC with rabbit, cow and human which could be conjugated to FITC using one of our EasyLink conjugation kits, like ab102885.

    https://www.abcam.com/cytokeratin-3-antibody-ae5-ab77869.html

    https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

    For cytokeratin 12, we do not carry any antibodies that have been tested in flow cytometry. However, if you would like to test ab124975 or ab113039 in FC, we could offer you a discount on a future purchase if you purchase, test, and submit an Abreview on the product (the discount is worth the price you pay for the antibody).

    For p63, we have an antibody conjugated to DyLight 488 that works in ICC/IF with human cells, ab139929. Again, if you would like to test this antibody in FC, we could offer you a future discount. https://www.abcam.com/p63-antibody-epr5701-dylight-488-ab139929.html

    For BCRP/ABCG2, we have 4 antibodies tested in FC, but non are conjugated to a fluorophore: ab24115, ab24114, and ab72788, and ab95692 (conjugated to biotin). You could use the purified antibody ab72788 with one of our conjugation kits to directly conjugate a fluorophore to the antibody.

    I hope this information helps. If you are interested in testing any of the antibodies as listed above, please let me know and I will set you up in our system.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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