Name: Anti-Keap1 antibody [OTI1B4]
SKU: ab119403
Rating: 4 (8 reviews)

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Western blot - Anti-Keap1 antibody [OTI1B4] (ab119403)

Key features and details

Mouse monoclonal [OTI1B4] to Keap1
Suitable for: Flow Cyt (Intra), WB, IHC-P
Reacts with: Mouse, Human, Recombinant fragment
Isotype: IgG1

产品名称

Anti-Keap1抗体[OTI1B4]
参阅全部 Keap1 一抗
描述

小鼠单克隆抗体[OTI1B4] to Keap1
宿主

Mouse
经测试应用

适用于: Flow Cyt (Intra), WB, IHC-Pmore details
种属反应性

与反应: Mouse, Human, Recombinant fragment
免疫原

Recombinant full length Human Keap1 produced in HEK293T cells (NP_987096).
阳性对照
- HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Keap1 overexpress plasmid; Jurkat and HeLa cells; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues.
常规说明

Dilute in PBS (pH7.3) before use.

This product was changed from ascites to tissue culture supernatant on 6^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
纯度

Protein G purified
纯化说明

ab119403 was purified from TCS by affinity chromatography.
克隆

单克隆
克隆编号

OTI1B4
同种型

IgG1
研究领域
- Neuroscience
- Processes

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
- Hep G2 nuclear extract lysate (ab14660)
Recombinant Protein
- Recombinant Human Keap1 protein (Tagged) (ab132384)

The Abpromise guarantee

Abpromise™承诺保证使用ab119403于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB	(3)	1/500 - 1/2000. Predicted molecular weight: 70 kDa.
IHC-P	(1)	1/150.

说明
Flow Cyt (Intra) 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/150.

功能

Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
组织特异性

Broadly expressed, with highest levels in skeletal muscle.
序列相似性

Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
结构域

The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
翻译后修饰

Ubiquitinated and subject to proteasomal degradation.
细胞定位

Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Target information above from: UniProt accession Q14145 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Omim: 606016 Human
- SwissProt: Q14145 Human
- SwissProt: Q9Z2X8 Mouse
- Unigene: 465870 Human
- Unigene: 248266 Mouse
别名
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
- Keap 1 antibody
- KEAP1 antibody
- KEAP1_HUMAN antibody
- Kelch like ECH associated protein 1 antibody
- Kelch like family member 19 antibody
- Kelch like protein 19 antibody
- Kelch-like ECH-associated protein 1 antibody
- Kelch-like protein 19 antibody
- KIAA0132 antibody
- KLHL 19 antibody
- KLHL19 antibody
- MGC10630 antibody
- MGC1114 antibody
- MGC20887 antibody
- MGC4407 antibody
- MGC9454 antibody
see all

Western blot - Anti-Keap1 antibody [OTI1B4] (ab119403)

All lanes : Anti-Keap1 antibody [OTI1B4] (ab119403) at 1/500 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.

Predicted band size: 70 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
Western blot - Anti-Keap1 antibody [OTI1B4] (ab119403)

All lanes : Anti-Keap1 antibody [OTI1B4] (ab119403) at 1/500 dilution

Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 70 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [OTI1B4] (ab119403)

ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [OTI1B4] (ab119403)

ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [OTI1B4] (ab119403)

ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
Flow Cytometry (Intracellular) - Anti-Keap1 antibody [OTI1B4] (ab119403)

ab119403 at 1/100 dilution staining Keap1 in HEK293T cells transfected with either pCMV6-ENTRY Keap1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry (Intracellular).
Flow Cytometry (Intracellular) - Anti-Keap1 antibody [OTI1B4] (ab119403)

ab119403 at 1/100 dilution staining Keap1 in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry (Intracellular) - Anti-Keap1 antibody [OTI1B4] (ab119403)

ab119403 at 1/100 dilution staining Keap1 in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab119403？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab119403 被引用在 30 文献中.

Gu X et al. 1,25(OH)2D3 ameliorates doxorubicin‑induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress. Exp Ther Med 26:413 (2023). PubMed: 37559932
Luffarelli R et al. Interferon Gamma Enhances Cytoprotective Pathways via Nrf2 and MnSOD Induction in Friedreich's Ataxia Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37628866
Zhang H et al. DL-3-n-butylphthalide (NBP) alleviates poststroke cognitive impairment (PSCI) by suppressing neuroinflammation and oxidative stress. Front Pharmacol 13:987293 (2022). PubMed: 36712684
Chen Y et al. Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress. Front Pharmacol 13:857932 (2022). PubMed: 35462905
Gao J et al. Oxymatrine inhibits the pyroptosis in rat insulinoma cells by affecting nuclear factor kappa B and nuclear factor (erythroid-derived 2)-like 2 protein/heme oxygenase-1 pathways. Korean J Physiol Pharmacol 26:165-174 (2022). PubMed: 35477544

View all Publications for this product

客户评价及客户问答

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提交评价提交问题

1-9 of 9 Abreviews or Q&A

Application

Western blot

Sample

Rat Tissue lysate - whole (Kidney)

Gel Running Conditions

Reduced Denaturing (12% gel)

Loading amount

20 µg

Specification

Kidney

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

勇貴前田

Verified customer

提交于 Jan 25 2023

Application

Western blot

Sample

Human Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (4-20% polyacrilamide gel Wet transfer at 400mA for 90 min)

Loading amount

15 µg

Specification

Skeletal muscle

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 10 2022

Application

Immunoprecipitation

Sample

Human Tissue lysate - whole (SHSY5Y)

Total protein in input

500000 cells

Treatment

0.15mM Diamide for 1h

Immuno-precipitation step

Protein A/G

Specification

SHSY5Y

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 02 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (lung)

Permeabilization

Specification

lung

Blocking step

Ventanas blocking kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (lungs)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Venta cell conditioner

Permeabilization

Specification

lungs

Blocking step

Ventanas blocking kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 20 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (C42B Prostate cancer cell line)

Permeabilization

Yes - 0.1% Triton X-100

Specification

C42B Prostate cancer cell line

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

提交于 Dec 13 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (C42B Prostate cancer cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

C42B Prostate cancer cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

提交于 May 14 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (prostate)

Permeabilization

Yes - triton

Specification

prostate

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 28 2014

Question

Dear Sirs,

I've bought recently Keap1 monoclonal antibody (catalogue number ab119403).

I’ve tried it on paraffin-embedded bladder and breast cancer tissue at the concentration suggested by your datasheet 1/150, but it didn’t worked.

I’ve also tried with and without Citrate Buffer pH6 antigen retrieval (10+10 minutes in the microwave), Peroxidase blocking for 7 minutes and I increased the concentration to 1/100 for 60 minutes, but I still get very mild staining, not at all comparable to the one you showed in the data sheet.

Could you please let me know any suggestion? Different antigen retrieval or no antigen retrieval, different concentration or different blocking time?

Thank you for your assistance.

Best regards,

Abcam community

Verified customer

Asked on Jul 12 2012

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC-P and inhuman and mouse. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:

General Information
Antibody storage conditions (temperature/reconstitution etc)

Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)

Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)

Antigen retrieval (Enzymatic method, Heat mediated technique etc.)

Permeabilization step

Blocking conditions (Buffer/time period, Blocking agent etc.)

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Detection method

Positive and negative controls used (please specify)

Optimization attempts (problem solving)
How many times have you tried the IHC?

Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No

What steps have you altered?

Additional Notes

We would appreciate if you are also able to provide and image which woudl help us to assess the results

Abcam Scientific Support

回复于 Jul 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

结构域

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (30)

客户评价及客户问答

Western blot abreview for Anti-Keap1 antibody [OTI1B4]

Western blot abreview for Anti-Keap1 antibody [OTI1B4]

Immunoprecipitation abreview for Anti-Keap1 antibody [OTI1B4]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Keap1 antibody [OTI1B4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Keap1 antibody [OTI1B4]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Keap1 antibody [OTI1B4]

Western blot abreview for Anti-Keap1 antibody [OTI1B4]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Keap1 antibody [OTI1B4]