Anti-Keap1抗体(ab226997)
Key features and details
- Rabbit polyclonal to Keap1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Keap1抗体
参阅全部 Keap1 一抗 -
描述
兔多克隆抗体to Keap1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Chimpanzee
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免疫原
Recombinant fragment within Human Keap1 (C terminal). The exact sequence is proprietary.
Database link: Q14145 -
阳性对照
- WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226997于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
|
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. -
组织特异性
Broadly expressed, with highest levels in skeletal muscle. -
序列相似性
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
结构域
The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5. -
翻译后修饰
Ubiquitinated and subject to proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 455706 Chimpanzee
- Entrez Gene: 532791 Cow
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Entrez Gene: 100136900 Pig
- Entrez Gene: 117519 Rat
- Omim: 606016 Human
- SwissProt: Q14145 Human
see all -
别名
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
图片
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Western blot - Anti-Keap1 antibody (ab226997)Anti-Keap1 antibody (ab226997) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 70 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Keap1 antibody (ab226997)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Keap1 (green) using ab226997 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab226997 被引用在 4 文献中.
- Jeon SB et al. Enhancement of porcine in vitro embryonic development through luteolin-mediated activation of the Nrf2/Keap1 signaling pathway. J Anim Sci Biotechnol 14:148 (2023). PubMed: 38037099
- Guo J et al. Hydrogen saline water accelerates fracture healing by suppressing autophagy in ovariectomized rats. Front Endocrinol (Lausanne) 13:962303 (2022). PubMed: 36120426
- Zhang X et al. Inhibition of thioredoxin-interacting protein may enhance the therapeutic effect of dehydrocostus lactone in cardiomyocytes under doxorubicin stimulation via the inhibition of the inflammatory response. Exp Ther Med 23:226 (2022). PubMed: 35222703
- Xu D et al. The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62. Sci Adv 5:eaav4570 (2019). PubMed: 31032410