Anti-KDS抗体(ab70297)

Thank you for taking the time to complete our questionnaire as requested.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. Could you confirm if the wash steps? I can suggest to wash in TBS containing 0.2% Tween if this has not already been tried. TBS can provide a more stringent wash than PBS and the Tween will help to wash away any excess antibody.

2. We recommend not to mix blocking agents in one experiment. Try BSA only.

3. Is the secondary antibody and detection kit working well with other primary antibodies? What are the results from the no primary control? This would help to assess if the secondary antibody is binding non specifically. The concentration of secondary antibody may need to be reduced in order to help optimize the results.

4. I am sorry to letyou know that the attachment with the image has not come through. I would appreciate if you are able to send this again.

5, I have found several slightly different recipes for EIA buffer. Could you confirm which recipe you have used? I can suggest to consider trying RIPA lysis buffer instead which may provide a more suitable protein preparation in this case.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for taking the time to contact us and for your telephone call yesterday afternoon. I appreciate your sending a message, I did in factunfortunately have your email address incorrectand am pleased to have confirmation of this.

I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB and human. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

As discussed on the phone,we would like to investigate thiscase further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

Thank you for providing an image alreadywhich would help us to assess the results. I will be away from the office next week, but one of my colleagues will be able to investigate this for you and get back to you.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.