Anti-KDM6A / UTX抗体(ab36938)
Key features and details
- Rabbit polyclonal to KDM6A / UTX
- Suitable for: ChIP, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-KDM6A / UTX抗体
参阅全部 KDM6A / UTX 一抗 -
描述
兔多克隆抗体to KDM6A / UTX -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab36938 are tested in WB. Previous batches were additionally validated in ChIP and ICC/IF. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab253183.
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经测试应用
适用于: ChIP, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human KDM6A/ UTX aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab36937) -
阳性对照
- WB: HeLa and HAP1 cell lysates; Whole NIH3T3 cell lysate (no shRNA and Control shRNA) ICC: MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36938于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP |
Use at an assay dependent concentration. PubMed: 26306033
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| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 154 kDa.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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ChIP
Use at an assay dependent concentration. PubMed: 26306033 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 154 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A. -
序列相似性
Belongs to the UTX family.
Contains 1 JmjC domain.
Contains 8 TPR repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7403 Human
- Entrez Gene: 22289 Mouse
- Entrez Gene: 317178 Rat
- Omim: 300128 Human
- SwissProt: O15550 Human
- SwissProt: O70546 Mouse
- Unigene: 522616 Human
- Unigene: 257498 Mouse
see all -
别名
- bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) antibody
- bA386N14.2 antibody
- bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX antibody
see all
图片
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Western blot - Anti-KDM6A / UTX antibody (ab36938)All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KDM6A knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 154 kDa
Observed band size: 154 -157 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab36938 observed at 154 -157 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab36938 was shown to react with KDM6A / UTX in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265110 (knockout cell lysate ab257214) was used. Wild-type HeLa and KDM6A knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab36938 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-KDM6A / UTX antibody (ab36938)ICC/IF image of ab36938 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab36938, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa cells fixed in 4% PFA at 1ug/ml and HeLa and MCF7 100% methanol fixed cells at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data. -
Western blot - Anti-KDM6A / UTX antibody (ab36938)Lanes 1-2 : Anti-KDM6A / UTX antibody (ab36938) at 0.7 µg/ml
Lanes 3-4 : Anti-Vinculin antibody [SPM227] (ab18058) at 1/20000 dilution
Lanes 1 & 3 : Wild-type HAP1 whole cell lysate
Lanes 2 & 4 : KDM6A knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 154 kDaLanes 1 - 2: Green - ab36938 observed at 154 kDa.
Lanes 3 - 4: Red - loading control, ab18058, observed at 130 kDa.
ab36938 was shown to recognize KDM6A in wild-type HAP1 cells as signal was lost at the expected MW in KDM6A knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM6A knockout samples were subjected to SDS-PAGE. Ab36938 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-KDM6A / UTX antibody (ab36938)This image is courtesy of an anonymous AbreviewAll lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/ml
Lane 1 : Whole NIH3T3 cell lysate (no shRNA)
Lane 2 : Whole NIH3T3 cell lysate (Control shRNA)
Lane 3 : Whole NIH3T3 cell lysate (Utx shRNA)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 154 kDa
Observed band size: 148 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsLane 3 is NIH3T3 cells transduced with lentivirus expressing shRNA for mouse Utx.
5% milk was used as the blocking agent (incubated for 1 hour at 25°C).
ab36938 was diluted with TBS+0.1% Tween 20 and incubated for 1 hour at 25°C.
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Western blot - Anti-KDM6A / UTX antibody (ab36938)All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/ml
Lane 1 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG -H&L- Pre Adsorbed (HRP)
at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 154 kDa
Observed band size: 154,157 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
This Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab36938 被引用在 33 文献中.
- Guo T et al. KDM6B interacts with TFDP1 to activate P53 signaling in regulating mouse palatogenesis. Elife 11:N/A (2022). PubMed: 35212626
- Houfaf Khoufaf FZ et al. Role of UTX Histone Demethylase in Regulation of MGMT, TRA2A, U2AF1, and RPS6KA2 Genes in Prostate Cancer Cell Lines. OMICS 25:129-131 (2021). PubMed: 33337267
- Kim CY et al. HOXA5 confers tamoxifen resistance via the PI3K/AKT signaling pathway in ER-positive breast cancer. J Cancer 12:4626-4637 (2021). PubMed: 34149926
- Ding Y et al. Kaposi's sarcoma-associated herpesvirus promotes mesenchymal-to-endothelial transition by resolving the bivalent chromatin of PROX1 gene. PLoS Pathog 17:e1009847 (2021). PubMed: 34492084
- Chen YH et al. Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study. BMC Cancer 21:979 (2021). PubMed: 34465286