重组Anti-KDM5B / PLU1 / Jarid1B抗体[EPR12704] (ab181089)
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.cn/ps/products/181/ab181089/Images/ab181089-213089-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg "Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-KDM5B / PLU1 / Jarid1B抗体[EPR12704]
参阅全部 KDM5B / PLU1 / Jarid1B 一抗 -
描述
兔单克隆抗体[EPR12704] to KDM5B / PLU1 / Jarid1B -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: BxPC-3, MCF7, and HeLa whole cell lysate (ab150035). Mouse and rat testes tissue lysates; ICC/IF: HeLa cells; Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12704 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181089于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). |
靶标
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功能
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. -
组织特异性
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). -
序列相似性
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
结构域
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10765 Human
- Entrez Gene: 75605 Mouse
- Entrez Gene: 304809 Rat
- Omim: 605393 Human
- SwissProt: Q9UGL1 Human
- SwissProt: Q80Y84 Mouse
- Unigene: 443650 Human
- Unigene: 28995 Mouse
see all -
别名
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
see all
图片
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Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.cn/ps/products/181/ab181089/Images/ab181089-295062-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.
ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.cn/ps/products/181/ab181089/Images/ab181089-213087-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.cn/ps/products/181/ab181089/Images/ab181089-268956-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + T47-D (human ductal carcinoma epithelia) whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.cn/ps/products/181/ab181089/Images/ab181089-268957-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.cn/ps/products/181/ab181089/Images/ab181089-8-benefits-of-recombinant-antibodies.png)
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (15)
ab181089 被引用在 15 文献中.
- Yang Y et al. Knockdown of KDM5B Leads to DNA Damage and Cell Cycle Arrest in Granulosa Cells via MTF1. Curr Issues Mol Biol 45:3219-3237 (2023). PubMed: 37185734
- Wang B et al. Loss of KDM5B ameliorates pathological cardiac fibrosis and dysfunction by epigenetically enhancing ATF3 expression. Exp Mol Med 54:2175-2187 (2022). PubMed: 36481938
- Tran TA et al. Inhibition of Jumonji demethylases reprograms severe dilated cardiomyopathy and prolongs survival. J Biol Chem 298:101515 (2022). PubMed: 34933013
- Zhou B et al. Hypoxia Stimulates SUMOylation-Dependent Stabilization of KDM5B. Front Cell Dev Biol 9:741736 (2021). PubMed: 34977006
- Guo JC et al. KDM5B promotes self-renewal of hepatocellular carcinoma cells through the microRNA-448-mediated YTHDF3/ITGA6 axis. J Cell Mol Med 25:5949-62 (2021). PubMed: 33829656