Anti-KDM3B / JMJD1B抗体(ab70797)
Key features and details
- Rabbit polyclonal to KDM3B / JMJD1B
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KDM3B / JMJD1B抗体
参阅全部 KDM3B / JMJD1B 一抗 -
描述
兔多克隆抗体to KDM3B / JMJD1B -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
Database link: Q7LBC6 -
阳性对照
- HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma
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常规说明
The immunogen used for this product shares 76% homology with KDM3A/JMJD1A. Cross-reactivity with this protein has not been confirmed experimentally.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70797 was affinity purified using an epitope specific to KDM3B / JMJD1B immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70797于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. -
组织特异性
Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver. -
序列相似性
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain. -
结构域
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51780 Human
- Entrez Gene: 277250 Mouse
- Omim: 609373 Human
- SwissProt: Q7LBC6 Human
- SwissProt: Q6ZPY7 Mouse
- Unigene: 483486 Human
- Unigene: 479894 Mouse
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别名
- 5qNCA antibody
- C5orf7 antibody
- JHDM2B antibody
see all
图片
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Western blot - Anti-KDM3B / JMJD1B antibody (ab70797)All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 191 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 30 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody (ab70797)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-KDM3B / JMJD1B antibody (ab70797)Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab70797 被引用在 4 文献中.
- Xie S et al. miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway. iScience 24:103271 (2021). PubMed: 34761190
- Zepp M et al. IDK1 is a rat monoclonal antibody against hypoglycosylated bone sialoprotein with application as biomarker and therapeutic agent in breast cancer skeletal metastasis. J Pathol Clin Res 4:55-68 (2018). PubMed: 29416877
- Liu Y et al. An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal cancer. Gut : (2012). Human . PubMed: 22345654
- Zheng Z et al. 5-Aza-2'-deoxycytidine reactivates gene expression via degradation of pRb pocket proteins. FASEB J 26:449-59 (2012). PubMed: 21990374