Anti-KDM3A / JHDM2A抗体(ab80598)
Key features and details
- Rabbit polyclonal to KDM3A / JHDM2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KDM3A / JHDM2A抗体
参阅全部 KDM3A / JHDM2A 一抗 -
描述
兔多克隆抗体to KDM3A / JHDM2A -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80598于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 147 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 147 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. -
序列相似性
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain. -
结构域
The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors. -
细胞定位
Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa. - Information by UniProt
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数据库链接
- Entrez Gene: 55818 Human
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
- Unigene: 557425 Human
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别名
- DKFZp686A24246 antibody
- DKFZp686P07111 antibody
- JHDM2A antibody
see all
图片
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Western blot - Anti-KDM3A / JHDM2A antibody (ab80598)All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Predicted band size: 147 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab80598 被引用在 4 文献中.
- Kong NR et al. Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression. Cell Rep 34:108574 (2021). PubMed: 33406418
- Xu Q et al. Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia. J Cell Mol Med 25:8127-8139 (2021). PubMed: 34350711
- Ho JC et al. Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway. PLoS One 12:e0188051 (2017). PubMed: 29145444
- Hickok JR et al. Nitric oxide modifies global histone methylation by inhibiting Jumonji C domain-containing demethylases. J Biol Chem 288:16004-15 (2013). WB ; Human . PubMed: 23546878