Anti-KDM2A抗体(ab174693)
Key features and details
- Goat polyclonal to KDM2A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-KDM2A抗体
参阅全部 KDM2A 一抗 -
描述
山羊多克隆抗体to KDM2A -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Hamster, Dog, Pig, Monkey
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免疫原
Synthetic peptide corresponding to Human KDM2A aa 445-459 (internal sequence) (Cysteine residue).
Sequence:PRKDRQVHLTHFELE
Database link: NP_036440.1 -
阳性对照
- Human small intestine tissue; Human uterus lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174693于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. -
组织特异性
Widely expressed, with highest levels in brain, testis and ovary, followed by lung. -
序列相似性
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
结构域
The JmjC domain mediates demethylation activity and is required for satellite silencing.
The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2. - Information by UniProt
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数据库链接
- Entrez Gene: 483702 Dog
- Entrez Gene: 100052955 Horse
- Entrez Gene: 22992 Human
- Entrez Gene: 225876 Mouse
- Entrez Gene: 100294703 Pig
- Entrez Gene: 361700 Rat
- Omim: 605657 Human
- SwissProt: Q9Y2K7 Human
see all -
别名
- [Histone-H3]-lysine-36 demethylase 1A antibody
- CXXC-type zinc finger protein 8 antibody
- CXXC8 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody (ab174693)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling KDM2A with ab174693 at 5 µg/ml.
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Western blot - Anti-KDM2A antibody (ab174693)Anti-KDM2A antibody (ab174693) at 0.5 µg/ml + Human uterus lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab174693 被引用在 3 文献中.
- Cheng T et al. Knockdown of circGOLPH3 inhibits cell progression and glycolysis by targeting miR-145-5p/lysine demethylase 2A (KDM2A) axis in oral squamous cell carcinoma. Head Neck 45:225-236 (2023). PubMed: 36268878
- Wang Y et al. p300 Acetylates JHDM1A to inhibit osteosarcoma carcinogenesis. Artif Cells Nanomed Biotechnol 47:2891-2899 (2019). PubMed: 31307234
- Wang F et al. LINC00460 modulates KDM2A to promote cell proliferation and migration by targeting miR-342-3p in gastric cancer. Onco Targets Ther 11:6383-6394 (2018). PubMed: 30323616