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重组RabMAb

重组Anti-KDEL抗体[EPR12668] (ab176333)

Reviews (3) Submit a question References (21)
Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] (ab176333)
  • Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
  • Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)
  • Flow Cytometry (Intracellular) - Anti-KDEL antibody [EPR12668] (ab176333)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)
  • Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)
  • Anti-KDEL antibody [EPR12668] (ab176333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12668] to KDEL
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-KDEL抗体[EPR12668]
    参阅全部 KDEL 一抗

  • 描述

    兔单克隆抗体[EPR12668] to KDEL

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • 293T, MCF-7, Raji, HepG2, HeLa, Jurkat, and K562 cell lysates. MCF7 and HeLa cells. Paraffin-embedded Human heart and kidney tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.06% BSA, 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12668

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176333于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/50 - 1/350. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF (1)
1/100 - 1/250.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/50 - 1/350. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF
1/100 - 1/250.

靶标

  • 相关性

    The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

  • 细胞定位

    Endoplasmic reticulum

  • 别名

    • KDEL antibody
    • Lys Asp Glu Leu antibody

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] (ab176333)
    Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] (ab176333)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling KDEL with purified ab176333 at 1/200. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

    For negative control 1, ab150120 (Rabbit primary antibody) and anti-mouse secondary antibody were used and for negative control 2, ab7291 (Mouse primary antibody) and ab150077 (anti-rabbit secondary antibody) were used. 

  • Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
    Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
    All lanes : Anti-KDEL antibody [EPR12668] (ab176333) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : HeLa cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa

  • Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
    Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
    Lane 1 : Anti-P4HB antibody [EPR9498] (ab137119) at 1/5000 dilution
    Lanes 2 & 4 & 6 & 8 & 10 : Anti-KDEL antibody [EPR12668] (ab176333) at 1/5000 dilution
    Lane 3 : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution
    Lane 5 : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/5000 dilution
    Lane 7 : Anti-ERp57 antibody [EPR10678(B)] (ab154191) at 1/5000 dilution
    Lane 9 : Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/5000 dilution
    Lane 11 : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/5000 dilution

    Lanes 1-6 & 8-11 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 7 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 25 kDa


    Exposure time: 3 minutes


    ab176333 detected several bands which have similar MWs with some proteins containing carboxyl terminal KDEL motifs, including P4HB, GRP78 BiP, GRP94, PDIA6 and LEPRE1 (PMID: 25683117, PMID: 19741001, PMID: 22079671, PMID: 28648146, PMID: 22615817).  It indicates that ab176333 might recognize these proteins.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)

    Immunohistochemical analysis of paraffin-embedded human liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Flow Cytometry (Intracellular) - Anti-KDEL antibody [EPR12668] (ab176333)
    Flow Cytometry (Intracellular) - Anti-KDEL antibody [EPR12668] (ab176333)

    Overlay histogram showing 4% paraformaldehyde fixed HeLa cells labelling KDEL (red) with purified ab176333 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black). The blue line shows cells without incubation with primary antibody and secondary antibody. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)

    Immunohistochemical analysis of paraffin-embedded rat kidney sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
    Western blot - Anti-KDEL antibody [EPR12668] (ab176333)
    All lanes : Anti-KDEL antibody [EPR12668] (ab176333) at 1/10000 dilution

    Lane 1 : Raw264.7 whole cell lysate
    Lane 2 : C6 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 25 kDa



    Observed band size : 94,78,57,48

    Blocking/Diluting buffer 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)

    Immunohistochemical analysis of paraffin-embedded mouse liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Anti-KDEL antibody [EPR12668] (ab176333)
    Anti-KDEL antibody [EPR12668] (ab176333)

文献 (21)

发表研究结果有使用 ab176333？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab176333 被引用在 21 文献中.

  • Sun S  et al. Spatial position is a key determinant of N-glycan functionality of the scavenger receptor cysteine-rich domain of human hepsin. FEBS J 290:3966-3982 (2023). PubMed: 36802168
  • Zhang J  et al. HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation. Invest Ophthalmol Vis Sci 64:5 (2023). PubMed: 37266953
  • Longfield SF  et al. Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles. Nat Commun 14:7277 (2023). PubMed: 37949856
  • Malik A  et al. Interleukin-10 Mitigates Doxorubicin-Induced Endoplasmic Reticulum Stress as Well as Cardiomyopathy. Biomedicines 10:N/A (2022). PubMed: 35453640
  • Yan X  et al. Myocilin Gene Mutation Induced Autophagy Activation Causes Dysfunction of Trabecular Meshwork Cells. Front Cell Dev Biol 10:900777 (2022). PubMed: 35615698
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-KDEL antibody [EPR12668] - ER Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% TritonX100 in PBS, 3mins
Specification
HeLa
Blocking step
2%BSA, 2% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Michiel Krols

Verified customer

提交于 Apr 27 2016

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