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重组RabMAb

重组Anti-KCNN3/SK3抗体[EPR28322-1] - BSA and Azide free (ab317692)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
  • Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28322-1] to KCNN3/SK3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-KCNN3/SK3抗体[EPR28322-1] - BSA and Azide free
    参阅全部 KCNN3/SK3 一抗

  • 描述

    兔单克隆抗体[EPR28322-1] to KCNN3/SK3 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-Pmore details
    不适用于: ICC/IF,IHC-Fr,IP or WB

  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse brain, Rat cerebrum, Mouse colon, Rat colon and HEK-293T transfected with a KCNN3 expression vector containing a his tag tissues. Flow Cyt( Intra): Mouse primary neuron and Rat primary neuron cells.

  • 常规说明

    ab317692 is the carrier-free version of ab317691.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317692于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF,IHC-Fr,IP or WB.

靶标

  • 功能

    Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.

  • 序列相似性

    Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • hSK3 antibody
      • KCa2.3 antibody
      • Kcnn3 antibody
      • KCNN3_HUMAN antibody
      • Potassium calcium activated channel subfamily N member 3 antibody
      • Potassium channel calcium activated intermediate/small conductance subfamily N alpha member 3 antibody
      • Potassium channel, calcium activated, intermediate/small conductance, subfamily N, member 3 antibody
      • Potassium intermediate/small conductance calcium activated channel subfamily N member 3 antibody
      • SK channel antibody
      • SK3 antibody
      • SKCa 3 antibody
      • SKCa3 antibody
      • Small conductance calcium-activated potassium channel protein 3 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling KCNN3/SK3 with ab317691 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse brain.
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling KCNN3/SK3 with ab317691 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on rat cerebrum (PMID: 12427825).
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling KCNN3/SK3 with ab317691 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on myenteric plexus of mouse colon.
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling KCNN3/SK3 with ab317691 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on myenteric plexus of rat colon.
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a KCNN3 expression vector containing a his tag. (B) HEK-293T transfected with a KCNN1 expression vector containing a his tag. (C) HEK-293T transfected with a KCNN2 expression vector containing a his tag. (D) HEK-293T transfected with empty vector containing a his tag. tissue labeling KCNN3/SK3 with ab317691 at 1/4000 (0.128 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      This antibody does not cross-react with human KCNN1 and KCNN2.
      Positive staining on (A) HEK-293T transfected with a KCNN3 expression vector containing a his tag. No staining on (B) HEK-293T transfected with KCNN1 expression vector containing a his tag, (C) HEK-293T transfected with KCNN2 expression vector containing a his tag and (D) HEK-293T transfected with empty vector containing a his tag.
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling KCNN3/SK3 with ab317691 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: No staining on mouse liver.
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling KCNN3/SK3 with ab317691 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: No staining on rat liver.
      The section was incubated with ab317691 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling KCNN3/SK3 with ab317691 at 1/500 dilution (0.1ug) / Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse spleen cell cells labelling KCNN3/SK3 with ab317691 at 1/500 dilution (0.1ug) / Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Low expression: spleen

    • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells labelling KCNN3/SK3 with ab317691 at 1/500 dilution (0.1ug) / Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Flow Cytometry (Intracellular) - Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

      This data was developed using ab317691, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat spleen cell cells labelling KCNN3/SK3 with ab317691 at 1/500 dilution (0.1ug) / Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Low expression: spleen

    • Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)
      Anti-KCNN3/SK3 antibody [EPR28322-1] - BSA and Azide free (ab317692)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317692？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317692 尚未被引用在任何文献中。

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