Anti-KCNN2/SK2抗体(ab99457)
Key features and details
- Goat polyclonal to KCNN2/SK2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KCNN2/SK2抗体
参阅全部 KCNN2/SK2 一抗 -
描述
山羊多克隆抗体to KCNN2/SK2 -
宿主
Goat -
特异性
Recognizes both Human isoforms (NP_067627.2 and NP_740721.1) -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human liver lysate. IHC-P: Human thyroid tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99457于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml.
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| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 404177 Cow
- Entrez Gene: 474640 Dog
- Entrez Gene: 3781 Human
- Entrez Gene: 140492 Mouse
- Entrez Gene: 100172000 Orangutan
- Entrez Gene: 54262 Rat
- Omim: 605879 Human
- SwissProt: Q6PJI0 Human
see all -
别名
- Apamin sensitive small conductance Ca2+ activated potassium antibody
- Apamin sensitive small conductance Ca2+ activated potassium channel antibody
- hSK2 antibody
see all
图片
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Western blot - Anti-KCNN2/SK2 antibody (ab99457)Anti-KCNN2/SK2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 64, 26 kDa
Observed band size: 23,70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab99457 被引用在 1 文献中.
- Du H et al. The Cation Channel TMEM63B Is an Osmosensor Required for Hearing. Cell Rep 31:107596 (2020). PubMed: 32375046
