Anti-KCC2抗体(ab97502)
Key features and details
- Rabbit polyclonal to KCC2
- Suitable for: ICC/IF, WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-KCC2抗体
参阅全部 KCC2 一抗 -
描述
兔多克隆抗体to KCC2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Cow, Dog, Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following rat tissue lysates: brain; cortex; hippocampus.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa). |
靶标
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功能
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. -
组织特异性
Brain specific. Detected in neuronal cells. -
序列相似性
Belongs to the SLC12A transporter family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 57468 Human
- Entrez Gene: 57138 Mouse
- Entrez Gene: 171373 Rat
- Omim: 606726 Human
- SwissProt: Q9H2X9 Human
- SwissProt: Q91V14 Mouse
- SwissProt: Q63633 Rat
- Unigene: 21413 Human
see all -
别名
- Electroneutral potassium chloride cotransporter 2 antibody
- Electroneutral potassium-chloride cotransporter 2 antibody
- Erythroid K Cl cotransporter 2 antibody
see all
图片
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Western blot - Anti-KCC2 antibody (ab97502)All lanes : Anti-KCC2 antibody (ab97502) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Cortex Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 126 kDa
Additional bands at: 250 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody (ab97502)ICC/IF image of ab97502 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97502, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97502 被引用在 2 文献中.
- Lam P et al. Beta-Amyloid (Aβ1-42) Increases the Expression of NKCC1 in the Mouse Hippocampus. Molecules 27:N/A (2022). PubMed: 35458638
- Xia W et al. The TWIST1-centered competing endogenous RNA network promotes proliferation, invasion, and migration of lung adenocarcinoma. Oncogenesis 8:62 (2019). PubMed: 31645542