AB249501

重组Anti-KBTBD11抗体[EPR10056(2)(B)] - BSA and Azide free

Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal KBTBD11 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

CMLAP, KIAA0711, KLHDC7C, KBTBD11, Kelch repeat and BTB domain-containing protein 11, Chronic myelogenous leukemia-associated protein, Kelch domain-containing protein 7B

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling KBTBD11 with ab169551 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.

ab169551 showing +ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.

ab169551 showing +ve staining in Human prostate hyperplasia tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.

ab169551 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling KBTBD11 with ab169551 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.Immunoprecipitation. ab169551 at 1/1000 staining KBTBD11 in fetal brain lysate immunoprecipitated using ab169551 at 1/10. Predicted band size : 66 kDa

All lanes:

Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (<a href='/products/primary-antibodies/kbtbd11-antibody-epr100562b-ab169551'>ab169551</a>)

Predicted band size: 66 kDa

false

Unknown

Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free (AB249501)

This data was developed using ab169551, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (<a href='/products/primary-antibodies/kbtbd11-antibody-epr100562b-ab169551'>ab169551</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

HT-29 lysate at 10 µg

Lane 4:

Caco-2 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-KBTBD11 antibody [EPR10056(2)(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10056(2)(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab249501 is the carrier-free version of ab169551.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KBTBD11 also known as Kelch Repeat and BTB (POZ) Domain-Containing Protein 11 is a protein with a molecular mass of approximately 70 kDa. This protein is part of the BTB-Kelch family which are characterized by a BTB protein-protein interaction domain and Kelch repeats that form a beta-propeller structure. KBTBD11 is expressed in various tissues with higher expression seen in the brain and testis indicating a role in neural activities and reproductive processes.

Biological function summary

KBTBD11 participates in the ubiquitin-proteasome system where it acts as a substrate adaptor for Cullin-RING E3 ligases (CRLs). By facilitating the ubiquitination of target proteins KBTBD11 regulates protein turnover and stability. It does not function independently and is part of a larger CRL complex that includes various other scaffolding and substrate recognition proteins. Through these interactions KBTBD11 impacts cellular processes such as signal transduction and cell cycle progression.

Pathways

KBTBD11 is involved in the ubiquitination and proteasome degradation pathways critical for maintaining proteostasis within cells. It shares functional relationships with other components like Cullin-3 which form an E3 ligase complex important for tagging damaged or unneeded proteins for degradation. This regulatory mechanism plays a significant role in controlling signal transduction pathways including cell growth and apoptotic processes in which protein degradation must be swiftly and accurately regulated.

KBTBD11 links to neurodegenerative diseases and cancers due to its role in protein degradation. Misregulation of its function can lead to an accumulation of ubiquitinated proteins which is a hallmark of neurodegenerative conditions such as Alzheimer’s disease. It may also be involved in cancer through pathways associated with cell cycle control where its malfunction can contribute to uncontrolled cell proliferation. In these contexts proteins like p53 are often critical in mediating the pathways impacted by KBTBD11 dysfunction.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information KBTBD11

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-KBTBD11抗体[EPR10056(2)(B)] - BSA and Azide free