Anti-KATNAL2抗体(ab184829)
Key features and details
- Rabbit polyclonal to KATNAL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-KATNAL2抗体
参阅全部 KATNAL2 一抗 -
描述
兔多克隆抗体to KATNAL2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human KATNAL2 aa 150-300.
Database link: Q8IYT4 -
阳性对照
- IHC-P: Human thyroid gland, testis, fallopian tube, cerebral cortex and skeletal muscle tissue; WB: A-549 and Caco-2 whole cell lysate; ICC: U-2 OS whole cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184829于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa.
|
|
IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
说明 |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
靶标
-
功能
Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays. -
序列相似性
Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily. A-like 2 sub-subfamily.
Contains 1 LisH domain. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 83473 Human
- Entrez Gene: 71206 Mouse
- Entrez Gene: 307253 Rat
- SwissProt: Q8IYT4 Human
- SwissProt: Q9D3R6 Mouse
- Unigene: 404137 Human
- Unigene: 35803 Mouse
-
别名
- DKFZP667C165 antibody
- Katanin p60 ATPase-containing subunit A-like 2 antibody
- Katanin p60 subunit A like 2 antibody
see all
图片
-
Immunohistochemical analysis of human thyroid gland labeling KATNAL2 in the cytoplasm of glandular cells with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-KATNAL2 antibody (ab184829) at 0.4 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Predicted band size: 61 kDaCorresponding KATNAL2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
-
Immunofluorescence analysis of hU-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling KATNAL2 in the nucleoplasm and intermediate filaments with ab184829 at 2 µg/ml.
-
Immunohistochemical analysis of human testis tissue labeling KATNAL2 in the cytoplasm and membrane of seminiferous ducts with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of human fallopian tube tissue labeling KATNAL2 in the cytoplasm of glandular cells with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of human cerebral cortex tissue labeling KATNAL2 in the cytoplasm of neurons with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of human skeletal muscle tissue labeling KATNAL2 in the cytoplasm of myocytes with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab184829 尚未被引用在任何文献中。