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重组RabMAb

重组Anti-KAT7 / Hbo1 / MYST2抗体[EPR7194(B)] (ab124993)

Submit a review Q&A (1)References (2)
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
  • Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
  • OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
  • Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-KAT7 / Hbo1 / MYST2抗体[EPR7194(B)]
    参阅全部 KAT7 / Hbo1 / MYST2 一抗

  • 描述

    兔单克隆抗体[EPR7194(B)] to KAT7 / Hbo1 / MYST2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human KAT7/ Hbo1/ MYST2 aa 50-150. The exact sequence is proprietary.
    Database link: O95251

  • 阳性对照

    • HeLa, Jurkat, MCF7, and HepG2 whole cell lysate (ab7900); Permeabilized HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124993于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.

  • 组织特异性

    Ubiquitously expressed, with highest levels in testis.

  • 序列相似性

    Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.

  • 结构域

    The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
    The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus > nucleoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Hbo 1 antibody
    • HBO1 antibody
    • HBOa antibody
    • Histone acetyltransferase binding to hORC1 antibody
    • Histone acetyltransferase binding to ORC1 antibody
    • Histone acetyltransferase KAT7 antibody
    • Histone acetyltransferase MYST2 antibody
    • K(lysine) acetyltransferase 7 antibody
    • KAT 7 antibody
    • KAT7 antibody
    • Lysine acetyltransferase 7 antibody
    • MOZ antibody
    • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
    • MYST 2 antibody
    • MYST histone acetyltransferase 2 antibody
    • MYST protein 2 antibody
    • MYST-2 antibody
    • MYST2 antibody
    • MYST2_HUMAN antibody
    • SAS 2 antibody
    • SAS2 and TIP60 protein 2 antibody
    • TIP60 protein 2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC7 antibody
    see all

图片

  • Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
    Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
    All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 71 kDa

  • Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
    Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)

    ab124993 at 1/100 dilution staining KAT7 / Hbo1 / MYST2 in permeabilized HeLa cells by intracellular flow cytometry (red) or negative control (green).

     

  • OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
    OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)
    Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993)

文献 (2)

发表研究结果有使用 ab124993？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124993 被引用在 2 文献中.

  • AlAbdi L  et al. Oct4-Mediated Inhibition of Lsd1 Activity Promotes the Active and Primed State of Pluripotency Enhancers. Cell Rep 30:1478-1490.e6 (2020). PubMed: 32023463
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359

客户评价及客户问答

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Question

might test ab31506 in xenopus, send testing discount code

xenopus: NP001079995, check homology
check homology of other Abs as well

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Answer

Thank you for your phone call.
The overall homology between Human and Xenopus is 93%. Thus, in general there is good chance for an antibody raised against human to work in Xenopus.
For ab31506 the homology of the immunogen with Xenopus is 100%.
For ab87100 the homology of the immunogen with Xenopus is 90%. For the other Hbo1 antibodies I currently do not have information about the immunogen range. I can check with the lab if you like - please let me know.
None of these antibodies has been tested in IP.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab31506 in Xenopus and IP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Xenopus/IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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