Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-KAT7 / Hbo1 / MYST2抗体(ab70183)

Submit a review Q&A (1)References (14)
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
  • Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)

Key features and details

  • Rabbit polyclonal to KAT7 / Hbo1 / MYST2
  • Suitable for: ELISA, IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-KAT7 / Hbo1 / MYST2抗体
    参阅全部 KAT7 / Hbo1 / MYST2 一抗

  • 描述

    兔多克隆抗体to KAT7 / Hbo1 / MYST2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, IHC-P, WB, ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human KAT7/ Hbo1/ MYST2 (internal sequence).
    Database link: O95251
    (Peptide available as ab182013)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab70183于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/100 - 1/500.
IP
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/100 - 1/500.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.

  • 组织特异性

    Ubiquitously expressed, with highest levels in testis.

  • 序列相似性

    Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.

  • 结构域

    The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
    The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus > nucleoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Hbo 1 antibody
    • HBO1 antibody
    • HBOa antibody
    • Histone acetyltransferase binding to hORC1 antibody
    • Histone acetyltransferase binding to ORC1 antibody
    • Histone acetyltransferase KAT7 antibody
    • Histone acetyltransferase MYST2 antibody
    • K(lysine) acetyltransferase 7 antibody
    • KAT 7 antibody
    • KAT7 antibody
    • Lysine acetyltransferase 7 antibody
    • MOZ antibody
    • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
    • MYST 2 antibody
    • MYST histone acetyltransferase 2 antibody
    • MYST protein 2 antibody
    • MYST-2 antibody
    • MYST2 antibody
    • MYST2_HUMAN antibody
    • SAS 2 antibody
    • SAS2 and TIP60 protein 2 antibody
    • TIP60 protein 2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC7 antibody
    see all

图片

  • Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 71 kDa
    Observed band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    HUVEC cells with ab70183 at 1/500 dilution, with and without the immunising peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    IHC image of ab70183 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献 (14)

发表研究结果有使用 ab70183？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab70183 被引用在 14 文献中.

  • Zhang FF  et al. The circular RNA Rap1b promotes Hoxa5 transcription by recruiting Kat7 and leading to increased Fam3a expression, which inhibits neuronal apoptosis in acute ischemic stroke. Neural Regen Res 18:2237-2245 (2023). PubMed: 37056143
  • Xie M  et al. Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis. Exp Mol Med 53:572-590 (2021). PubMed: 33811247
  • MacPherson L  et al. HBO1 is required for the maintenance of leukaemia stem cells. Nature 577:266-270 (2020). PubMed: 31827282
  • Khan O  et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature 571:211-218 (2019). PubMed: 31207603
  • Long C  et al. LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells. Biochim Biophys Acta Gene Regul Mech 1861:773-782 (2018). PubMed: 30745998
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

might test ab31506 in xenopus, send testing discount code

xenopus: NP001079995, check homology
check homology of other Abs as well

Read More
Answer

Thank you for your phone call.
The overall homology between Human and Xenopus is 93%. Thus, in general there is good chance for an antibody raised against human to work in Xenopus.
For ab31506 the homology of the immunogen with Xenopus is 100%.
For ab87100 the homology of the immunogen with Xenopus is 90%. For the other Hbo1 antibodies I currently do not have information about the immunogen range. I can check with the lab if you like - please let me know.
None of these antibodies has been tested in IP.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab31506 in Xenopus and IP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Xenopus/IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com