Anti-KAT13C / NCOA2抗体(ab10491)

False colour image of Western blot: Anti-KAT13C / NCOA2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab10491 was shown to bind specifically to KAT13C / NCOA2. A band was observed at 160 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in NCOA2 knockout cell line ab265068 (knockout cell lysate ab258530). To generate this image, wild-type and NCOA2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

ab10491 used for WB at 0.1 µg/ml (A) 

Lane 1: Whole cell lysate Hela, 50 ug

Lane 2: Whole cell lysate Hela, 15 ug

Lane 3: Whole cell lysate HEK293T, 50 ug

Lane 4: Whole cell lysate Jurkat, 50 ug

ab10491 was used at 1 µg/ml for WB and used for IP at 6 µg/mg lysate (B)

Lane 1-3: ab10491]

Lane4: Control IgG

Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).