Anti-KAT13A / SRC1抗体(ab5407)
Key features and details
- Rabbit polyclonal to KAT13A / SRC1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KAT13A / SRC1抗体
参阅全部 KAT13A / SRC1 一抗 -
描述
兔多克隆抗体to KAT13A / SRC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 600-650.
Database link: Q15788 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000.
|
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| IHC-P |
1/500 - 1/2000.
|
|
| IP | (1) |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. |
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IHC-P
1/500 - 1/2000. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. -
组织特异性
Widely expressed. -
疾病相关
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children. -
序列相似性
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro. -
翻译后修饰
Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8648 Human
- Entrez Gene: 17977 Mouse
- Omim: 602691 Human
- SwissProt: Q15788 Human
- SwissProt: P70365 Mouse
- Unigene: 596314 Human
- Unigene: 301039 Mouse
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别名
- bHLHe74 antibody
- Class E basic helix-loop-helix protein 74 antibody
- F SRC 1 antibody
see all
图片
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Western blot - Anti-KAT13A / SRC1 antibody (ab5407)All lanes : Anti-KAT13A / SRC1 antibody (ab5407) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa cells at 50 µg
Lane 5 : Mouse NIH3T3 cells at 50 µgDetection:Chemiluminescence
Exposure time:30 seconds
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Immunoprecipitation - Anti-KAT13A / SRC1 antibody (ab5407)KAT13A/SRC1 was immunoprecipitated from 1mg of 293T cells prepared using NETN lysis buffer.
Exposure time: 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13A / SRC1 antibody (ab5407)Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma labeling KAT13A/SRC1 with ab5407 at 1/1000 dilution.
Detection: DAB.
Counter stained with Hematoxylin.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab5407 被引用在 12 文献中.
- Li S et al. Ginsenoside Rh2 suppresses colon cancer growth by targeting the miR-150-3p/SRCIN1/Wnt axis. Acta Biochim Biophys Sin (Shanghai) 55:633-648 (2023). PubMed: 36916297
- Ren ZL et al. Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations. Zool Res 43:442-456 (2022). PubMed: 35503560
- Zhang Y et al. miR-657 Targets SRCIN1 via the Slug Pathway to Promote NSCLC Tumor Growth and EMT Induction. Dis Markers 2022:4842454 (2022). PubMed: 36033827
- Pensieri P et al. Photoreceptor cKO of OTX2 Enhances OTX2 Intercellular Transfer in the Retina and Causes Photophobia. eNeuro 8:N/A (2021). PubMed: 34475267
- Zhao PW et al. SRC-1 and Twist1 are prognostic indicators of liver cancer and are associated with cell viability, invasion, migration and epithelial-mesenchymal transformation of hepatocellular carcinoma cells. Transl Cancer Res 9:603-612 (2020). PubMed: 35117405