Anti-KAT13A / SRC1抗体(ab2859)
Key features and details
- Rabbit polyclonal to KAT13A / SRC1
- Suitable for: ICC, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-KAT13A / SRC1抗体
参阅全部 KAT13A / SRC1 一抗 -
描述
兔多克隆抗体to KAT13A / SRC1 -
宿主
Rabbit -
特异性
Detects steroid receptor coactivator-1 (SRC-1). -
经测试应用
适用于: ICC, ICC/IF, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig
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免疫原
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阳性对照
- ICC: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate ICC/IF: A549 (Human lung carcinoma cell line) whole cell lysate IHC-Fr: Mouse brain ; Rat Brain
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
靶标
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功能
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. -
组织特异性
Widely expressed. -
疾病相关
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children. -
序列相似性
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro. -
翻译后修饰
Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8648 Human
- Entrez Gene: 17977 Mouse
- Entrez Gene: 574068 Pig
- Entrez Gene: 313929 Rat
- Omim: 602691 Human
- SwissProt: Q15788 Human
- SwissProt: P70365 Mouse
- SwissProt: Q4PJW2 Pig
see all -
别名
- bHLHe74 antibody
- Class E basic helix-loop-helix protein 74 antibody
- F SRC 1 antibody
see all
图片
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Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
Immunolocalization of SRC1 in HeLa cells using ab2859.
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Immunocytochemistry/ Immunofluorescence - Anti-KAT13A / SRC1 antibody (ab2859)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.25% Triton™ X-100 permeabilized, 70% confluent log phase A549 (Human lung carcinoma cell line) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling KAT13A / SRC1 with ab2859 at 1 µg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhangab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary. -
Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab2859 被引用在 9 文献中.
- Huber AD et al. Mutation of a single amino acid of pregnane X receptor switches an antagonist to agonist by altering AF-2 helix positioning. Cell Mol Life Sci 78:317-335 (2021). PubMed: 32232515
- Liu L et al. Confirmation of inhibitingTLR4/MyD88/NF-κB Signalling Pathway by Duhuo Jisheng Decoction on Osteoarthritis: A Network Pharmacology Approach-Integrated Experimental Study. Front Pharmacol 12:784822 (2021). PubMed: 35140604
- Zhou B et al. The Nuclear Orphan Receptor NR2F6 Promotes Hepatic Steatosis through Upregulation of Fatty Acid Transporter CD36. Adv Sci (Weinh) 7:2002273 (2020). PubMed: 33173745
- Castellaro AM et al. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 30736340
- Kong X et al. Roux-en-Y gastric bypass enhances insulin secretion in type 2 diabetes via FXR-mediated TRPA1 expression. Mol Metab 29:1-11 (2019). PubMed: 31668381
- Kong X et al. FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic ß-Cells. J Diabetes Res 2019:8915818 (2019). PubMed: 31485455
- Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . PubMed: 27842582
- Hurtado A et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP ; Human . PubMed: 19005469
- Yasumoto H et al. GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. J Cell Sci 120:2532-43 (2007). WB . PubMed: 17623774