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Immunology Immunoglobulins Light Chain Kappa

Anti-Kappa light chain抗体[MEM-09] (ab1050)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [MEM-09] to Kappa light chain
  • Suitable for: IHC-P, ICC, Flow Cyt (Intra), IP, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Kappa light chain抗体[MEM-09]
    参阅全部 Kappa light chain 一抗
  • 描述

    小鼠单克隆抗体[MEM-09] to Kappa light chain
  • 宿主

    Mouse
  • 特异性

    MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM.
  • 经测试应用

    适用于: IHC-P, ICC, Flow Cyt (Intra), IP, WB, Flow Cytmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Kappa light chain. Crude thymus membrane fraction

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from TCS. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    MEM-09
  • 同种型

    IgG1
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Kappa

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1050于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • 细胞定位

    Cytoplasmic and Secreted
  • 数据库链接

    • Entrez Gene: 28901 Human
    • Entrez Gene: 3514 Human
    • Omim: 147200 Human
    • SwissProt: P01601 Human
    • SwissProt: P01834 Human
    • 别名

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    实验方案

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (4)

    发表研究结果有使用 ab1050?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1050 被引用在 4 文献中.

    • Cao J  et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). PubMed: 29311542
    • Shah KA  et al. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng 112:2624-9 (2015). PubMed: 26032261
    • O'Callaghan PM  et al. Cell line-specific control of recombinant monoclonal antibody production by CHO cells. Biotechnol Bioeng 106:938-51 (2010). WB ; Chinese hamster . PubMed: 20589672
    • Horejsi­ V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Kappa light chain antibody [MEM-09]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (B lymphocytes)
    Loading amount
    15 µg
    Specification
    B lymphocytes
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 06 2008

    Question

    which dilution do you use for mem09 used in WB??? always 1ug/ml

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2002

    Answer

    Since the immunoglobulin concentration of this product is 1mg/ml, I would suggest a starting concentration of 1ug/ml. Adjust this accordingly from the results obtained.

    Read More

    Abcam Scientific Support

    回复于 Aug 07 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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