Anti-Kappa light chain抗体[MEM-09] (ab1050)
Key features and details
- Mouse monoclonal [MEM-09] to Kappa light chain
- Suitable for: IHC-P, ICC, Flow Cyt (Intra), IP, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Kappa light chain抗体[MEM-09]
参阅全部 Kappa light chain 一抗 -
描述
小鼠单克隆抗体[MEM-09] to Kappa light chain -
宿主
Mouse -
特异性
MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM. -
经测试应用
适用于: IHC-P, ICC, Flow Cyt (Intra), IP, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Kappa light chain. Crude thymus membrane fraction
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from TCS. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-09 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
|
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WB | (1) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
细胞定位
Cytoplasmic and Secreted -
数据库链接
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
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别名
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab1050 被引用在 4 文献中.
- Cao J et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). PubMed: 29311542
- Shah KA et al. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng 112:2624-9 (2015). PubMed: 26032261
- O'Callaghan PM et al. Cell line-specific control of recombinant monoclonal antibody production by CHO cells. Biotechnol Bioeng 106:938-51 (2010). WB ; Chinese hamster . PubMed: 20589672
- Horejsi V et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063