AB1050

Anti-Kappa light chain 抗体 [MEM-09]

Name: Kappa light chain抗体[MEM-09] (ab1050)| Abcam中文官网
Brand: Abcam Limited
SKU: AB1050
Price: 4864 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Kappa light chain antibody [MEM-09]

(1 Review)

(4 Publications)

Mouse Monoclonal IGKC antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Tissue preparation containing IGKC protein.

查看别名

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

1 Images

Flow Cytometry - Anti-Kappa light chain antibody [MEM-09] (AB1050)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Kappa light chain antibody [MEM-09] (AB1050)

Flow cytometric analysis of human peripheral whole blood stained using ab1050 (concentration in sample 3 µg/ml) GAM APC showing separation of human Ig Kappa Light Chain positive lymphocytes (red-filled) from Ig Kappa Light Chain negative lymphocytes (black-dashed).

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-09

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

IHC-P, Flow Cyt (Intra), ICC, IP, WB, Flow Cyt

applications

免疫原

Tissue preparation containing IGKC protein. The exact immunogen used to generate this antibody is proprietary information.

P01834

特异性

MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1-5 µg/mL", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Purified from TCS. Purity >95% by SDS-PAGE.

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:77 PubMed29311542

2018

Versatile and on-demand biologics co-production in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Jicong Cao,Pablo Perez-Pinera,Ky Lowenhaupt,Ming-Ru Wu,Oliver Purcell,Cesar de la Fuente-Nunez,Timothy K Lu

Biotechnology and bioengineering 112:2624-9 PubMed26032261

2015

Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris.

Applications

Unspecified application

Species

Unspecified reactive species

Kartik A Shah,John J Clark,Brittany A Goods,Timothy J Politano,Nicholas J Mozdzierz,Ross M Zimnisky,Rachel L Leeson,J Christopher Love,Kerry R Love

Biotechnology and bioengineering 106:938-51 PubMed20589672

2010

Cell line-specific control of recombinant monoclonal antibody production by CHO cells.

Applications

Species

Chinese hamster

Peter M O'Callaghan,Jane McLeod,Leon P Pybus,Clare S Lovelady,Stephen J Wilkinson,Andrew J Racher,Alison Porter,David C James

Folia biologica 32:12-25 PubMed2422063

1986

Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,I Hilgert,H Kristofová,V Bazil,A Bukovský,J Kulhánková

View all publications

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Anti-Kappa light chain 抗体 [MEM-09]

Anti-Kappa light chain antibody [MEM-09]

Flow Cytometry - Anti-Kappa light chain antibody [MEM-09] (AB1050)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性