重组Anti-Kappa light chain抗体[EPR5367-8] (ab124727)
![ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.cn/ps/products/124/ab124727/Images/ab124727-8-anti-kappa-light-chain-antibody-epr5367-8-elisa.jpg "ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5367-8] to Kappa light chain
- Suitable for: WB, IP, ELISA, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Kappa light chain抗体[EPR5367-8]
参阅全部 Kappa light chain 一抗 -
描述
兔单克隆抗体[EPR5367-8] to Kappa light chain -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ELISA, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.
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阳性对照
- IP: Human spleen lysate. WB: Human tonsil, plasma and spleen lysate; IHC: Human tonsil lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5367-8 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124727于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
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| IP |
1/10 - 1/100.
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| ELISA |
1/16000.
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 25 kDa. |
|
IP
1/10 - 1/100. |
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ELISA
1/16000. |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
靶标
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相关性
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
细胞定位
Cytoplasmic and Secreted -
数据库链接
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
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别名
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
图片
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ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.cn/ps/products/124/ab124727/Images/ab124727-7-anti-kappa-light-chain-antibody-epr5367-8-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.cn/ps/products/124/ab124727/Images/ab124727-458205-anti-kappa-light-chain-antibody-epr5367-8-westernblot-human.jpg)
Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)All lanes : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution (Purified)
Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.cn/ps/products/124/ab124727/Images/ab124727-5-anti-kappa-light-chain-antibody-epr5367-8-immunoprecipitation-spleen-human.jpg)
Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input): Human spleen lysate 10µg
Lane 2 (+): ab124727 + Human spleen lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa -
![Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.cn/ps/products/124/ab124727/Images/ab124727-3-benefits-of-recombinant-antibodies.png)
Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124727 被引用在 2 文献中.
- Flisikowska T et al. A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies. Nat Biomed Eng 6:1248-1256 (2022). PubMed: 36138193
- Deng Z et al. Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33760139