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Immunology Immunoglobulins Light Chain Kappa

Anti-Kappa light chain抗体[4G7] (ab1936)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [4G7] (ab1936)

    Key features and details

    • Mouse monoclonal [4G7] to Kappa light chain
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

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    概述

    • 产品名称

      Anti-Kappa light chain抗体[4G7]
      参阅全部 Kappa light chain 一抗
    • 描述

      小鼠单克隆抗体[4G7] to Kappa light chain
    • 宿主

      Mouse
    • 特异性

      Specific for independent constantly expressed epitope of kappa chain. Reacts with free and heavy chain-associated kappa chains.
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Full length protein corresponding to Human Kappa light chain.

    • 阳性对照

      • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
    • 常规说明

      Concentration varies from lot to lot and can be provided on request.

      This product was changed from ascites to tissue culture supernatant in March 2015. Lot numbers higher than GR145316-1 will be from tissue culture supernatant.  Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      4G7
    • 骨髓瘤

      Sp2/0
    • 同种型

      IgG2a
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Light Chain
      • Kappa

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1936于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 相关性

      Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
    • 细胞定位

      Cytoplasmic and Secreted
    • 数据库链接

      • Entrez Gene: 28901 Human
      • Entrez Gene: 3514 Human
      • Omim: 147200 Human
      • SwissProt: P01601 Human
      • SwissProt: P01834 Human
      • 别名

        • HCAK 1 antibody
        • HCAK1 antibody
        • Ig kappa chain C region antibody
        • IGKC antibody
        • IGKCD antibody
        • Immunoglobulin InV antibody
        • Immunoglobulin kappa constant antibody
        • Immunoglobulin kappa constant region antibody
        • Immunoglobulin kappa light chain antibody
        • Immunoglobulin kappa light chain constant region antibody
        • Immunoglobulin KM antibody
        • Kappa 1 immunoglobulin light chain antibody
        • kappa light chain antibody
        • Km antibody
        • MGC111575 antibody
        • MGC62011 antibody
        • MGC72072 antibody
        • MGC88770 antibody
        • MGC88771 antibody
        • MGC88809 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [4G7] (ab1936)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [4G7] (ab1936)

        IHC image of Kappa light chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1936, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab1936?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1936 被引用在 2 文献中.

      • De Franceschi N  et al. Selective integrin endocytosis is driven by interactions between the integrin a-chain and AP2. Nat Struct Mol Biol 23:172-9 (2016). PubMed: 26779610
      • Rintanen N  et al. Calpains promote a2ß1 integrin turnover in nonrecycling integrin pathway. Mol Biol Cell 23:448-63 (2012). PubMed: 22160595

      客户评价及客户问答

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      1-5 of 5 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Kappa light chain antibody [4G7]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (human glioblastoma cell line U87MG)
      Specification
      human glioblastoma cell line U87MG
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 1% Triton X 100 in PBS
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 06 2012

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Kappa light chain antibody [4G7]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (glioblastoma cell line from rat)
      Specification
      glioblastoma cell line from rat
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 1% Triton X 100 in PBS
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 0.5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 06 2012

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Kappa light chain antibody [4G7]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (human glioblastoma cell line)
      Specification
      human glioblastoma cell line
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0,1% Triton X 100 in PBS
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 23 2012

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Kappa light chain antibody [4G7]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (glioblastoma cell line C6)
      Specification
      glioblastoma cell line C6
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0,1% Triton X 100 in PBS
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 23 2012

      Question

      I recently purchased this product and have found it to be useful. Consequently I was hoping that you could confirm the epitope sequence that this antibody binds to (clone 4G7)?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 28 2006

      Answer

      Thank you for your enquiry. We have not mapped the epitope for this antibody. Please do not hesitate to contact us if you have additional questions.

      Read More

      Abcam Scientific Support

      回复于 Aug 30 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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