AB1936

Anti-Kappa light chain抗体[4G7]

Name: Kappa light chain抗体[4G7] (ab1936)| Abcam中文官网
Brand: Abcam Limited
SKU: AB1936
Rating: 5 (4 reviews)

Anti-Kappa light chain antibody [4G7]

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(4 Reviews)

(3 Publications)

Mouse Monoclonal KVD16 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Human IGKV1D-16.

查看别名

Immunoglobulin kappa variable 1D-16, Ig kappa chain V-I region HK146, Ig kappa chain V-I region HK189, IGKV1D-16

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [4G7] (AB1936)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [4G7] (AB1936)

IHC image of Kappa light chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1936, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4G7

亚型

IgG2a

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

Full Length Protein corresponding to Human IGKV1D-16. The exact immunogen used to generate this antibody is proprietary information.

P01601

特异性

Specific for independent constantly expressed epitope of kappa chain. Reacts with free and heavy chain-associated kappa chains.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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产品详情

Concentration varies from lot to lot and can be provided on request.

This product was changed from ascites to tissue culture supernatant in March 2015. Lot numbers higher than GR145316-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed : 24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKV1D-16

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics & chromatin 18:58 PubMed40855509

2025

The isoflavone genistein selectively stimulates major satellite repeat transcription in mouse heterochromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Fuhrmann,Nicholas Shukeir,Reagan W Ching,Galina Erikson,Yuan Dou,Zoe Sawitzki,Megumi Onishi-Seebacher,Carmen Galan,Thomas Jenuwein

Nature structural & molecular biology 23:172-9 PubMed26779610

2016

Selective integrin endocytosis is driven by interactions between the integrin α-chain and AP2.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola De Franceschi,Antti Arjonen,Nadia Elkhatib,Konstantin Denessiouk,Antoni G Wrobel,Thomas A Wilson,Jeroen Pouwels,Guillaume Montagnac,David J Owen,Johanna Ivaska

Molecular biology of the cell 23:448-63 PubMed22160595

2011

Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Rintanen,Mikko Karjalainen,Jonna Alanko,Lassi Paavolainen,Anita Mäki,Liisa Nissinen,Moona Lehkonen,Katri Kallio,R Holland Cheng,Paula Upla,Johanna Ivaska,Varpu Marjomäki

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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Anti-Kappa light chain抗体[4G7]

Anti-Kappa light chain antibody [4G7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [4G7] (AB1936)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性