AB133440

重组Anti-KAP1 (phospho S824)抗体[EPR5248]

Name: KAP1(磷酸化S824)抗体[EPR5248] (ab133440)| Abcam中文官网
Brand: Abcam Limited
SKU: AB133440
Price: 3161 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-KAP1 (phospho S824) antibody [EPR5248]

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(14 Publications)

Rabbit Recombinant Monoclonal KAP1 phospho S824 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Synthetic peptide samples. Cited in 14 publications.

查看别名

KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

5 Images

Lab

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

Purified ab133440 at 1/50 dilution (2μg) immunoprecipitating KAP1 in HeLa treated with 3uM etoposide for 1h whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 3uM etoposide for 1h whole cell lysate 10μg
Lane 2 (+) : ab133440 + HeLa treated with 3uM etoposide for 1h whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32132 in HeLa treated with 3uM etoposide for 1h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 110 kDa

All lanes:

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

Predicted band size: 88 kDa

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Lab

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Wild type HAP1 + DMSO whole cell lysate (20 μg)
Lane 3 : Wild type HAP1 + Bleomycin whole cell lysate (20 μg)
Lane 4 : KAP1 knockout HAP1 whole cell lysate (20 μg)
Lane 5 : KAP1 knockout HAP1 + DMSO whole cell lysate (20 μg)
Lane 6 : KAP1 knockout HAP1 + Bleomycin whole cell lysate (20 μg)
Lane 7 : HeLa + DMSO whole cell lysate (20 μg)
Lane 8 : HeLa + Bleomycin whole cell lysate (20 μg)

Lanes 1 - 8 : Merged signal (red and green). Green - ab133440 observed at 105 kDa. Red - loading control, ab130007, observed at 125 kDa.

ab133440 was shown to specifically react with KAP1 in wild type cells as signal was lost in KAP1 knockout cells. Wild-type and KAP1 knockout samples were subjected to SDS-PAGE. ab133440 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C both at a 1/20000 dilution. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. Treated with 30 μg/mL Bleomycin in DMSO for 30 minutes.

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

Predicted band size: 88 kDa

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Lab

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 5µM etoposide for 8 hours whole cell lysate at 20 µg

Lane 3:

HeLa treated with 5µM etoposide for 8 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 110 kDa

false

Exposure time: 7s

Unknown

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 3ÂµM etoposide for 1 hour whole cell lysate

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) treated with 3ÂµM etoposide for 1 hour whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 88 kDa

false

Unknown

Dot Blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

Dot blot analysis of KAP1 (phospho S824) phospho peptide (Lane 1) and KAP1 non-phospho peptide (Lane 2) labelling KAP1 (phospho S824) with Unpurified ab133440 at a dilution of 1/1000. A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20000. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

不同偶联物与剂型 (1)

Carrier free

Anti-KAP1 (phospho S824) antibody [EPR5248] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5248

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

WB, Dot, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.

Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed : 23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610).. (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.

See full target information TRIM28 phospho S824

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40842237

2025

Ints7 deficiency activates DNA damage response to elicit resurgence of endogenous retrovirus MERVL and anastasis of embryonic stem cells.

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Unspecified application

Species

Unspecified reactive species

Yunfan Shen,Li Li,Huiling Ni,Hui Li,Mingrui Xu,Xiaoyang Tan,Zhangjie Li,Pishun Li,Fang Chen,Song Mao,Gongping Sun,Kai Yuan

Nature communications 15:10435 PubMed39616195

2024

Multi-protomics analysis identified host cellular pathways perturbed by tick-borne encephalitis virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Sui,Wenfang Wang,Xuerui Guo,Yinghua Zhao,Tian Tian,Jinlong Zhang,Heming Wang,Yueshan Xu,Hongmiao Chi,Hanxi Xie,Wenbo Xu,Nan Liu,Li Zhao,Guangqi Song,Zedong Wang,Kaiyu Zhang,Lihe Che,Yicheng Zhao,Guoqing Wang,Quan Liu

Cell insight 3:100170 PubMed38590928

2024

LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability.

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Species

Unspecified reactive species

Xiaqing Zhang,Jing Guo,Xin Shi,Xin Zhou,Qiang Chen

Open biology 14:230407 PubMed38262603

2024

A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells.

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Species

Unspecified reactive species

Megan M Schmit,Ryan M Baxley,Liangjun Wang,Peter Hinderlie,Marissa Kaufman,Emily Simon,Anjali Raju,Jeffrey S Miller,Anja-Katrin Bielinsky

Nature cancer 4:181-202 PubMed36732634

2023

Integrative multi-omics networks identify PKCδ and DNA-PK as master kinases of glioblastoma subtypes and guide targeted cancer therapy.

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Unspecified application

Species

Unspecified reactive species

Simona Migliozzi,Young Taek Oh,Mohammad Hasanain,Luciano Garofano,Fulvio D'Angelo,Ryan D Najac,Alberto Picca,Franck Bielle,Anna Luisa Di Stefano,Julie Lerond,Jann N Sarkaria,Michele Ceccarelli,Marc Sanson,Anna Lasorella,Antonio Iavarone

EJNMMI research 12:50 PubMed35962885

2022

Radiofluorination of a highly potent ATM inhibitor as a potential PET imaging agent.

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Claudia Rose Fraser,Javier Ajenjo,Mathew Veal,Gemma Marie Dias,Chung Chan,Edward O'Neill,Gianluca Destro,Doreen Lau,Anna Pacelli,Veronique Gouverneur,Rebekka Hueting,Bart Cornelissen

Molecular cancer therapeutics 21:859-870 PubMed35405736

2022

A New Class of Selective ATM Inhibitors as Combination Partners of DNA Double-Strand Break Inducing Cancer Therapies.

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Species

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Astrid Zimmermann,Frank T Zenke,Li-Ya Chiu,Heike Dahmen,Ulrich Pehl,Thomas Fuchss,Thomas Grombacher,Beatrix Blume,Lyubomir T Vassilev,Andree Blaukat

Molecular cancer therapeutics 21:245-256 PubMed34911817

2021

RP-3500: A Novel, Potent, and Selective ATR Inhibitor that is Effective in Preclinical Models as a Monotherapy and in Combination with PARP Inhibitors.

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Unspecified reactive species

Anne Roulston,Michal Zimmermann,Robert Papp,Alexander Skeldon,Charles Pellerin,Émilie Dumas-Bérube,Valerie Dumais,Stéphane Dorich,Lee D Fader,Sara Fournier,Li Li,Marie-Eve Leclaire,Shou Yun Yin,Amandine Chefson,Hunain Alam,William Yang,Chloe Fugère-Desjardins,Sabrina Vignini-Hammond,Kathryn Skorey,Amina Mulani,Victoria Rimkunas,Artur Veloso,Martine Hamel,Rino Stocco,Yael Mamane,Zuomei Li,Jordan T F Young,Michael Zinda,W Cameron Black

Scientific reports 11:12148 PubMed34108527

2021

DNA-PK inhibitor peposertib enhances p53-dependent cytotoxicity of DNA double-strand break inducing therapy in acute leukemia.

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Species

Unspecified reactive species

Eric Haines,Yuki Nishida,Michael I Carr,Rafael Heinz Montoya,Lauren B Ostermann,Weiguo Zhang,Frank T Zenke,Andree Blaukat,Michael Andreeff,Lyubomir T Vassilev

British journal of cancer : PubMed32741974

2020

Complete loss of ATM function augments replication catastrophe induced by ATR inhibition and gemcitabine in pancreatic cancer models.

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Unspecified application

Species

Unspecified reactive species

Charles R Dunlop,Yann Wallez,Timothy Isaac Johnson,Sandra Bernaldo de Quirós Fernández,Stephen T Durant,Elaine B Cadogan,Alan Lau,Frances M Richards,Duncan I Jodrell

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