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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methylated DNA

Anti-KAP1抗体(ab10484)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (53)

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Western blot - Anti-KAP1 antibody (ab10484)
  • Immunoprecipitation - Anti-KAP1 antibody (ab10484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)

Key features and details

  • Rabbit polyclonal to KAP1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-KAP1 antibody [EPR5249] (ab109545)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-KAP1抗体
    参阅全部 KAP1 一抗
  • 描述

    兔多克隆抗体to KAP1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IP, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human KAP1 aa 650-750.
    Database link: Q13263

  • 阳性对照

    • WB: HeLa, 293T, 3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma, mouse plasmacytoma, mouse coronal brain tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies were affinity purified using the peptide immobilized on solid support.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methylated DNA
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
    • Epigenetics and Nuclear Signaling
    • Bromodomains

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human KAP1 protein (ab131899)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab10484于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
1/500 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).
IP (1)
Use at 2-10 µg/mg of lysate.
IHC-P (1)
Use a concentration of 2 µg/ml.
说明
WB
1/500 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).
IP
Use at 2-10 µg/mg of lysate.
IHC-P
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
  • 组织特异性

    Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • 通路

    Protein modification; protein sumoylation.
  • 序列相似性

    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 结构域

    The HP1 box is both necessary and sufficient for HP1 binding.
    The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
    The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
    Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.
  • 细胞定位

    Nucleus. Associated with centromeric heterochromatin during cell differentiation through CBX1.
  • Target information above from: UniProt accession Q13263 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10155 Human
    • Entrez Gene: 21849 Mouse
    • Entrez Gene: 116698 Rat
    • Omim: 601742 Human
    • SwissProt: Q13263 Human
    • SwissProt: Q62318 Mouse
    • SwissProt: O08629 Rat
    • Unigene: 467408 Human
    • Unigene: 15701 Mouse
    • Unigene: 398345 Mouse
    • Unigene: 198494 Rat
    see all
  • 别名

    • E3 SUMO protein ligase TRIM28 antibody
    • E3 SUMO-protein ligase TRIM28 antibody
    • FLJ29029 antibody
    • KAP 1 antibody
    • KAP-1 antibody
    • KRAB associated protein 1 antibody
    • KRAB interacting protein 1 antibody
    • KRAB-associated protein 1 antibody
    • KRAB-interacting protein 1 antibody
    • KRIP 1 antibody
    • KRIP-1 antibody
    • KRIP1 antibody
    • Nuclear corepressor KAP 1 antibody
    • Nuclear corepressor KAP-1 antibody
    • RING finger protein 96 antibody
    • RNF96 antibody
    • TF1B antibody
    • TIF1 beta antibody
    • TIF1-beta antibody
    • TIF1B antibody
    • TIF1B_HUMAN antibody
    • Transcription intermediary factor 1 beta antibody
    • Transcription intermediary factor 1-beta antibody
    • Trim28 antibody
    • Tripartite motif containing 28 antibody
    • tripartite motif containing protein 28 antibody
    • Tripartite motif-containing protein 28 antibody
    see all

图片

  • Western blot - Anti-KAP1 antibody (ab10484)
    Western blot - Anti-KAP1 antibody (ab10484)
    All lanes : Anti-KAP1 antibody (ab10484) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 100 kDa


    Exposure time: 1 second


    Cells prepared using NETN lysis buffer. Chemiluminescence detection.

  • Immunoprecipitation - Anti-KAP1 antibody (ab10484)
    Immunoprecipitation - Anti-KAP1 antibody (ab10484)

    CTCF was immunoprecipitated from 1 mg HeLa whole cell lysate with ab10484 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab10484 at 1 µg/mL.

    Lysates prepared using NETN lysis buffer.

    Lane 1: ab10484 IP in HeLa whole cell lysate.

    Lane 2: Contol IgG in HeLa whole cell lysate.

    Detection: Chemiluminescence.

    Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling KAP1 with ab10484 at 1/5000 dilution (0.2 μg/ml). DAB detection, Hematoxylin counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse plasmacytoma, labeling KAP1 with ab10484 at 1 µg/mL. Detection: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)Image courtesy of an anonymous Abreview.
    ab10484 staining KAP1 in murine coronal brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized using 1% Triton. Samples were then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at 2µg/ml for 16 hours at 4°C. An Alexa-Fluor 488-conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/1000 dilution.
    Dentate gyrus of the hippocampus immunostained with DAPI (top) and KAP1 (bottom), which nicely labels the nuclei of the entire dentate gyrus.

实验方案

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (53)

发表研究结果有使用 ab10484?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab10484 被引用在 53 文献中.

  • Nyberg WA  et al. The bromodomain protein TRIM28 controls the balance between growth and invasiveness in melanoma. EMBO Rep 24:e54944 (2023). PubMed: 36341538
  • Ao Y  et al. Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence. Cells 12:N/A (2023). PubMed: 36831305
  • Imamura R  et al. APTX acts in DNA double-strand break repair in a manner distinct from XRCC4. J Radiat Res 64:485-495 (2023). PubMed: 36940705
  • Kato M  et al. Long non-coding RNA lncMGC mediates the expression of TGF-β-induced genes in renal cells via nucleosome remodelers. Front Mol Biosci 10:1204124 (2023). PubMed: 37325470
  • Heath J  et al. POGZ promotes homology-directed DNA repair in an HP1-dependent manner. EMBO Rep 23:e51041 (2022). PubMed: 34758190
View all Publications for this product

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Immunoprecipitation abreview for Anti-KAP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa Cells)
Total protein in input
3 µg
Specification
HeLa Cells
Immuno-precipitation step
Protein A
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Aug 25 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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