重组Anti-Kallikrein 6抗体[EPR23352-86] - BSA and Azide free (Capture) (ab281189)
![Sandwich ELISA - Anti-Kallikrein 6 antibody [EPR23352-86] - BSA and Azide free (Capture) (ab281189)](https://www.abcam.cn/ps/products/281/ab281189/Images/ab281189-2-human-kallikrein-6-klk6-elisa-kit-sandwich-elisa-standard-curve-5.jpg "Sandwich ELISA - Anti-Kallikrein 6 antibody [EPR23352-86] - BSA and Azide free (Capture) (ab281189)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23352-86] to Kallikrein 6 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-Kallikrein 6抗体[EPR23352-86] - BSA and Azide free (Capture)
参阅全部 Kallikrein 6 一抗 -
描述
兔单克隆抗体[EPR23352-86] to Kallikrein 6 - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281189 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human KLK6. The recommended pair for sandwich ELISA is:
Capture: ab281189, Human KLK6 Capture Antibody (unconjugated)
Detector: ab281039, Human KLK6 Detector Antibody (unconjugated)
The reference range value is 7.8 - 500 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23352-86 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281189于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23352-111] to Kallikrein 6 - BSA and Azide free (Detector) (ab281039).
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23352-111] to Kallikrein 6 - BSA and Azide free (Detector) (ab281039). |
靶标
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功能
Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. -
组织特异性
In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors. -
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
翻译后修饰
Inactivated by autolytic cleavage after Arg-80. -
细胞定位
Secreted. Nucleus > nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress. - Information by UniProt
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数据库链接
- Entrez Gene: 5653 Human
- Omim: 602652 Human
- SwissProt: Q92876 Human
- Unigene: 79361 Human
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别名
- Bssp antibody
- hK 6 antibody
- hK6 antibody
see all
图片
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Sandwich ELISA - Anti-Kallikrein 6 antibody [EPR23352-86] - BSA and Azide free (Capture) (ab281189)Example of Human Kallikrein 6/KLK6 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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![Anti-Kallikrein 6 antibody [EPR23352-86] - BSA and Azide free (Capture) (ab281189)](https://www.abcam.cn/ps/products/281/ab281189/Images/ab281189-1-RecombinantAntibodyBenefitfigure.png)
Anti-Kallikrein 6 antibody [EPR23352-86] - BSA and Azide free (Capture) (ab281189)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281189 尚未被引用在任何文献中。
