重组Anti-Kallikrein 10/K10抗体[EPR23573-107] - BSA and Azide free (ab273883)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23573-107] to Kallikrein 10/K10 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Kallikrein 10/K10抗体[EPR23573-107] - BSA and Azide free
参阅全部 Kallikrein 10/K10 一抗 -
描述
兔单克隆抗体[EPR23573-107] to Kallikrein 10/K10 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: NIH:OVCAR-3 whole cell lysate.
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常规说明
ab273883 is the carrier-free version of ab273878.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23573-107 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273883于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
靶标
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功能
Has a tumor-suppressor role for NES1 in breast and prostate cancer. -
组织特异性
Expressed in breast, ovary and prostate. -
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
发展阶段
Down-regulated during breast cancer progression. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5655 Human
- Omim: 602673 Human
- SwissProt: O43240 Human
- Unigene: 275464 Human
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别名
- Breast normal epithelial cell associated serine protease antibody
- Kallikrein related peptidase 10 antibody
- Kallikrein-10 antibody
see all
图片
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All lanes : Anti-Kallikrein 10/K10 antibody [EPR23573-107] (ab273878) at 1/1000 dilution
Lane 1 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:12821340).
Negative control: MCF7 (PMID: 11691827).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer format containing PBS. glycerol, BSA and sodium azide (ab273878).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273883 尚未被引用在任何文献中。