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重组RabMAb

重组Anti-Kaiso抗体[EPR4668] (ab124777)

Submit a review Submit a question References (2)
Western blot - Anti-Kaiso antibody [EPR4668] (ab124777)
  • Anti-Kaiso antibody [EPR4668] (ab124777)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4668] to Kaiso
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Kaiso抗体[EPR4668]
    参阅全部 Kaiso 一抗

  • 描述

    兔单克隆抗体[EPR4668] to Kaiso

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Kaiso aa 600-700. The exact sequence is proprietary.

  • 阳性对照

    • WB: Human heart, MCF7, C6, PC12 or NIH 3T3 lysates

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124777于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa).
说明
WB
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.

  • 组织特异性

    Expressed in vascular endothelium.

  • 序列相似性

    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.

  • 细胞定位

    Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Kaiso antibody
      • Kaiso protein antibody
      • Kaiso transcription factor antibody
      • KAISO_HUMAN antibody
      • Transcriptional regulator Kaiso antibody
      • ZBTB 33 antibody
      • Zbtb33 antibody
      • Zinc finger and BTB domain containing 33 antibody
      • Zinc finger and BTB domain-containing protein 33 antibody
      • Zinc finger transcription factor Kaiso antibody
      • ZNF 348 antibody
      • ZNF Kaiso antibody
      • ZNF348 antibody
      see all

    图片

    • Western blot - Anti-Kaiso antibody [EPR4668] (ab124777)
      Western blot - Anti-Kaiso antibody [EPR4668] (ab124777)
      All lanes : Anti-Kaiso antibody [EPR4668] (ab124777) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : MCF7 lysate
      Lane 3 : C6 lysate
      Lane 4 : PC12 lysate
      Lane 5 : NIH 3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 74 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?

    • Anti-Kaiso antibody [EPR4668] (ab124777)
      Anti-Kaiso antibody [EPR4668] (ab124777)

    文献 (2)

    发表研究结果有使用 ab124777？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124777 被引用在 2 文献中.

    • Chen H  et al. Overexpression of miR-1283 Inhibits Cell Proliferation and Invasion of Glioma Cells by Targeting ATF4. Oncol Res 27:325-334 (2019). PubMed: 29716673
    • Wang L  et al. Kaiso (ZBTB33) Downregulation by Mirna-181a Inhibits Cell Proliferation, Invasion, and the Epithelial-Mesenchymal Transition in Glioma Cells. Cell Physiol Biochem 48:947-958 (2018). PubMed: 30036882

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