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Anti-Kaiso抗体[6F / 6F8] (ab12723)

Reviews (5)Q&A (3)References (20)
Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
  • Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
  • Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (ab12723)
  • Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (ab12723)
  • Western blot - Anti-Kaiso antibody [6F / 6F8] (ab12723)

Key features and details

  • Mouse monoclonal [6F / 6F8] to Kaiso
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Kaiso抗体[6F / 6F8]
    参阅全部 Kaiso 一抗

  • 描述

    小鼠单克隆抗体[6F / 6F8] to Kaiso

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WB, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Hamster, Dog

  • 免疫原

    Fusion protein corresponding to Human Kaiso.

  • 阳性对照

    • CSML0 cells (IP then Western). Flow Cyt (Intra): HeLa cells.

  • 常规说明

    We can conjugate this antibody to FITC for you (please see ab150256 for details).

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab12723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (2)
Use at an assay dependent concentration.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.

  • 组织特异性

    Expressed in vascular endothelium.

  • 序列相似性

    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.

  • 细胞定位

    Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Kaiso antibody
      • Kaiso protein antibody
      • Kaiso transcription factor antibody
      • KAISO_HUMAN antibody
      • Transcriptional regulator Kaiso antibody
      • ZBTB 33 antibody
      • Zbtb33 antibody
      • Zinc finger and BTB domain containing 33 antibody
      • Zinc finger and BTB domain-containing protein 33 antibody
      • Zinc finger transcription factor Kaiso antibody
      • ZNF 348 antibody
      • ZNF Kaiso antibody
      • ZNF348 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
      Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
      Immunofluorescence using clone 6F / 6F8 on C2C12 cells.
    • Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
      Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

      Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

      Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.
    • Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (ab12723)
      Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (ab12723)
      Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (ab12723)
      Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (ab12723)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.
      Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.
    • Western blot - Anti-Kaiso antibody [6F / 6F8] (ab12723)
      Western blot - Anti-Kaiso antibody [6F / 6F8] (ab12723)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.
      Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.

    文献 (20)

    发表研究结果有使用 ab12723？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab12723 被引用在 20 文献中.

    • Salam H  et al. Association of Kaiso and partner proteins in oral squamous cell carcinoma. J Taibah Univ Med Sci 18:802-811 (2023). PubMed: 36852243
    • Singhal SK  et al. Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival. Commun Biol 4:150 (2021). PubMed: 33526872
    • Tong W  et al. Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33576467
    • Hiramatsu S  et al. Regulation of Cathepsin E gene expression by the transcription factor Kaiso in MRL/lpr mice derived CD4+ T cells. Sci Rep 9:3054 (2019). PubMed: 30816218
    • Choi SH  et al. Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis. J Biol Chem 294:12957-12974 (2019). PubMed: 31296660
    View all Publications for this product

    客户评价及客户问答

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    1-8 of 8 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Kaiso [6F / 6F8] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Human Cell (SW480)
    Specification
    SW480
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 11 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Kaiso [6F / 6F8] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Mouse Cell (NIH 3T3)
    Specification
    NIH 3T3
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 11 2013

    Western blot abreview for Anti-Kaiso [6F / 6F8] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (sw480)
    Specification
    sw480
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 11 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Kaiso antibody [6F / 6F8] - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mice APC Min)
    Specification
    Mice APC Min
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris buffer
    Permeabilization
    No
    Blocking step
    1% gelatin 10%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Raul Peña

    Verified customer

    提交于 Dec 18 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Kaiso antibody [6F / 6F8] - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreas)
    Specification
    Pancreas
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 06 2009

    Question

    ab12723 is labeled as clone 6F/6F8. These are actually 2 different clones so the notation is confusing. Is this product a combination of the 2 different monoclonals?

    Read More
    Answer


    It seems that this antibody is sometimes referred to as 6F8 and sometimes as 6F. So we've labeled it with both clone names.

    Read More

    Question

    http://www.ncbi.nlm.nih.gov/pubmed/22974583

    Jones J, Wang H, Zhou J, Hardy S, Turner T, Austin D, He Q, Wells A, Grizzle WE, Yates C.

    Am J Pathol. 2012 Nov;181(5):1836-46. doi: 10.1016/j.ajpath.2012.08.008. Epub 2012 Sep 10.


    PMID:22974583 [PubMed - in process]

    Read More
    Answer

    Thank you for contacting Abcam and for letting us know that ab12723 was used in that publication. I have passed this information to our references team, who will review the article and then upload that reference to our website.
    If there is anything else I can help you with, please let me know.

    Read More

    Question

    The immunogen says, "His-tagged Kaiso fusion protein (Human) lacking the zinc finger region."  Do you have the actual residues?  According to Swiss Prot, the region of a.a. 514 - 638 is required for DNA binding.  Does this work in ChIP because this region is included in the immunogen?  

    Read More
    Answer

    Thank you for your inquiry. I heard back from our lab that unfortunately we do not have any more information on the immunogen sequence other than what is stated on the datasheet.    Ideally,  for an antibody that works in ChIP, the immunogen sequence should avoid the DNA binding domain.  This is because if the target protein has bound to the DNA, this region is now blocked and no longer accessible to the antibody.  For this reason we also try to avoid designing immunogens to the activation domain too.  I hope this information helps.  Please contact us with any other questions.  

    Read More

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