重组Anti-JunD (phospho S100) + c-Jun (phospho S73)抗体[EPR16586] (ab178858)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16586] to JunD (phospho S100) + c-Jun (phospho S73)
- Suitable for: Dot blot, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-JunD (phospho S100) + c-Jun (phospho S73)抗体[EPR16586]
参阅全部 JunD+c-Jun 一抗 -
描述
兔单克隆抗体[EPR16586] to JunD (phospho S100) + c-Jun (phospho S73) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, IP, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa untreated and treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysates; NIH/3T3 untreated and treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysates; RAW 264.7 and PC-12 whole cell lysates. IHC-P: Human tonsil, mouse testis and rat liver tissues. IP: HeLa treated with 250ng/ml Anisomycin for 30 minutes whole cell extract.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16586 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178858于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Dot blot |
1/1000.
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IP |
1/100.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 40,45,48 kDa (predicted molecular weight: 36 kDa).
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说明 |
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Dot blot
1/1000. |
IP
1/100. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 40,45,48 kDa (predicted molecular weight: 36 kDa). |
靶标
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细胞定位
JunD: Nucleus. c-Jun: Nucleus. -
数据库链接
- Entrez Gene: 3725 Human
- Entrez Gene: 3727 Human
- Entrez Gene: 16476 Mouse
- Entrez Gene: 16478 Mouse
- Entrez Gene: 24516 Rat
- Entrez Gene: 24518 Rat
- Omim: 165160 Human
- Omim: 165162 Human
see all
图片
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All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40,45,48 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).
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Dot blot analysis of JunD (phospho S100) + c-Jun (phospho S73) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Lanes 1 & 4-5 : Anti-c-Jun antibody [E254] - ChIP Grade (ab32137)
Lanes 2-3 : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution
Lanes 1 & 3 & 5 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lanes 2 & 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes, whole cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lane 2 : Untreated NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).
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All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes and endothelial cells of Human tonsil is observed. Counter stained with Hematoxylin.
Negative Control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on spermatogoniums and Leydig cells of mouse testis is observed. Counter stained with Hematoxylin.
Negative Control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on bile duct epithelial cells while no staining on hepatocytes of rat liver is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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JunD (phospho S100) + c-Jun (phospho S73) were immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma), treated with 250ng/ml Anisomycin for 30 minutes, whole cell extract with ab178858 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab178858 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa, treated with 250ng/ml Anisomycin for 30 minutes, whole cell extract
Lane 2: PBS.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab178858 被引用在 3 文献中.
- Pan J et al. Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution. Front Cell Dev Biol 9:758339 (2021). PubMed: 34805166
- Jatczak-Pawlik I et al. Nanoparticles for Directed Immunomodulation: Mannose-Functionalized Glycodendrimers Induce Interleukin-8 in Myeloid Cell Lines. Biomacromolecules 22:3396-3407 (2021). PubMed: 34286584
- Shao P et al. MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway. Am J Transl Res 9:4300-4307 (2017). PubMed: 28979703