重组Anti-JunD抗体[EPR17365]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] (AB181615)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling JunD with ab181615 at 1/10000 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
1. ab181615 at 1/10000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (AB181615)

Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (AB181615)

Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nuclear staining on the epithelial cells of Human mammary gland was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (AB181615)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nuclear staining on neurons of the rat cerebral cortex was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (AB181615)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nuclear staining on neurons of the mouse cerebral cortex was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-JunD antibody [EPR17365] (AB181615)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µg

Lane 3:

PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] (AB181615)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

Lane 1:

293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] (AB181615)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] (AB181615)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 5s