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Anti-JPH3抗体(ab79063)

Submit a review Q&A (1)References (1)
Western blot - Anti-JPH3 antibody (ab79063)
  • Immunocytochemistry/ Immunofluorescence - Anti-JPH3 antibody (ab79063)

Key features and details

  • Rabbit polyclonal to JPH3
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-JPH3抗体

  • 描述

    兔多克隆抗体to JPH3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    A synthetic 18 amino acid peptide near the carboxy terminus of human JPH3 (protein accession number: NP_065706)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab79063于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa.
说明
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa.

靶标

  • 功能

    Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic reticulum. May play an active role in certain neurons involved in motor coordination.

  • 组织特异性

    Specifically expressed in brain.

  • 疾病相关

    Defects in JPH3 are the cause of Huntington disease-like type 2 (HDL2) [MIM:606438]. Huntington disease (HD) is a neurodegenerative disorder resulting primarily from the loss of medium spiny projection neurons in the striatum, especially in the caudate nucleus, and, to a lesser extent, atrophy of mesencephalic and cortical structures. The typical clinical picture of HD combines familial adult onset chorea and subcortical dementia that usually begin during the fourth decade of life.

  • 序列相似性

    Belongs to the junctophilin family.
    Contains 8 MORN repeats.

  • 结构域

    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.

  • 细胞定位

    Cell membrane. Endoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • JP-3 antibody
      • JP3 antibody
      • JPH3 antibody
      • JPH3_HUMAN antibody
      • Junctophilin 3 antibody
      • Junctophilin type 3 antibody
      • Junctophilin-3 antibody
      • TNRC22 antibody
      • Trinucleotide repeat-containing gene 22 protein antibody
      see all

    图片

    • Western blot - Anti-JPH3 antibody (ab79063)
      Western blot - Anti-JPH3 antibody (ab79063)
      All lanes : Anti-JPH3 antibody (ab79063) at 1 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : Daudi cell lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 81 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-JPH3 antibody (ab79063)
      Immunocytochemistry/ Immunofluorescence - Anti-JPH3 antibody (ab79063)
      Immunofluorescence of JPH3 in Human Brain cells using ab79063 at 20 ug/ml.

    文献 (1)

    发表研究结果有使用 ab79063？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab79063 被引用在 1 文献中.

    • Huang Y  et al. Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC. Mol Med Rep 25:N/A (2022). PubMed: 35169860

    客户评价及客户问答

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    Question

    WB with mouse brain lysate
    sees 2 bands: 150 kDa, above 150 kDa
    Ab: 1/200 o/n
    block: 5% milk 1h RT
    2nd: OK
    lot: GR59486-1

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab81178. The estimated delivery date is xxx.

    To check the status of the order please contact our Customer Service team and reference this number.

    Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    Here is the diiscount code for trying the antibody ab81778 in mouse.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab81178 in mouse. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    I wish you the best of luck with your research.

    Read More

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