Anti-JNK1 (phospho T183)抗体(ab47337)
Key features and details
- Rabbit polyclonal to JNK1 (phospho T183)
- Suitable for: WB, ELISA, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-JNK1 (phospho T183)抗体
参阅全部 JNK1 一抗 -
描述
兔多克隆抗体to JNK1 (phospho T183) -
宿主
Rabbit -
特异性
The region of JNK1 surrounding T183 has a high degree of similarity to the corresponding regions in JNK2 and JNK3 and thus may cross react with these proteins if phosphorylated on the corresponding residues. -
经测试应用
适用于: WB, ELISA, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human JNK1 aa 150-250 (phospho T183). Swiss-Prot #: P45983/P45984/P53779
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23226105
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23226105 |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. - Information by UniProt
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数据库链接
- Entrez Gene: 5599 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 116554 Rat
- Omim: 601158 Human
- SwissProt: P45983 Human
- SwissProt: Q91Y86 Mouse
- SwissProt: P49185 Rat
- Unigene: 138211 Human
see all -
别名
- AI849689 antibody
- c Jun N terminal kinase 1 antibody
- C-JUN kinase 1 antibody
see all
图片
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Western blot - Anti-JNK1 (phospho T183) antibody (ab47337)All lanes : Anti-JNK1 (phospho T183) antibody (ab47337)
Lane 1 : Extract of Hela cells treated with Anisomycin (200ng/ml, 10min)
Lane 2 : Extract of Hela cells, untreated
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK1 (phospho T183) antibody (ab47337)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47337 at a 1/50 dilution.Left image : Un-treated
Right image : Treated with P-peptide
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (51)
ab47337 被引用在 51 文献中.
- Elshal M & Hazem SH Escin suppresses immune cell infiltration and selectively modulates Nrf2/HO-1, TNF-α/JNK, and IL-22/STAT3 signaling pathways in concanavalin A-induced autoimmune hepatitis in mice. Inflammopharmacology 30:2317-2329 (2022). PubMed: 36063304
- Zhu D et al. Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling. Diabetol Metab Syndr 13:89 (2021). PubMed: 34446088
- Tan HY et al. Downregulation of miR-223 promotes HMGB2 expression and induces oxidative stress to activate JNK and promote autophagy in an in vitro model of acute lung injury. J Inflamm (Lond) 18:29 (2021). PubMed: 34732212
- Bie C et al. Insulin-Like Growth Factor 1 Receptor Drives Hepatocellular Carcinoma Growth and Invasion by Activating Stat3-Midkine-Stat3 Loop. Dig Dis Sci N/A:N/A (2021). PubMed: 33559791
- Huang L et al. Epigenetic modulation of the MAPK pathway prevents isoflurane-induced neuronal apoptosis and cognitive decline in aged rats. Exp Ther Med 20:35 (2020). PubMed: 32952626