重组Anti-JNK1抗体[EPR140(2)] (ab110724)
![Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)](https://www.abcam.cn/ps/products/110/ab110724/Images/ab110724-503824-anti-jnk1-antibody-epr1402-western-blot-wildtype-u2-os-mapk8-knockout-u2-os.jpg "Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR140(2)] to JNK1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-JNK1抗体[EPR140(2)]
参阅全部 JNK1 一抗 -
描述
兔单克隆抗体[EPR140(2)] to JNK1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HEK-293, K562, C6, RAW 264.7 and MCF7 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR140(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110724于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
靶标
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功能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. - Information by UniProt
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数据库链接
- Entrez Gene: 5599 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 116554 Rat
- Omim: 601158 Human
- SwissProt: P45983 Human
- SwissProt: Q91Y86 Mouse
- SwissProt: P49185 Rat
- Unigene: 138211 Human
see all -
别名
- AI849689 antibody
- c Jun N terminal kinase 1 antibody
- C-JUN kinase 1 antibody
see all
图片
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Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : MAPK8 knockout U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 42-48 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-JNK1 antibody [EPR140(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110724 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)](https://www.abcam.cn/ps/products/110/ab110724/Images/ab110724-253301-anti-jnk1-antibody-epr1402-western-blot.jpg)
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.ab110724 (unpurified) was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)](https://www.abcam.cn/ps/products/110/ab110724/Images/ab110724-351367-anti-jnk1-antibody-epr140-2-westernblot-human-mouse-rat.jpg)
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/2000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)](https://www.abcam.cn/ps/products/110/ab110724/Images/ab110724-279976-anti-jnk1-antibody-epr1402-western-blot.jpg)
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green).Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.
This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.
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![Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)](https://www.abcam.cn/ps/products/110/ab110724/Images/ab110724-124016-anti-jnk1-antibody-epr1402-western-blot.jpg)
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa -
![Anti-JNK1 antibody [EPR140(2)] (ab110724)](https://www.abcam.cn/ps/products/110/ab110724/Images/ab110724-5-benefits-of-recombinant-antibodies.png)
Anti-JNK1 antibody [EPR140(2)] (ab110724)
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab110724 被引用在 37 文献中.
- Tian X et al. c-Jun N-terminal kinase 2 suppresses pancreatic cancer growth and invasion and is opposed by c-Jun N-terminal kinase 1. Cancer Gene Ther 29:73-86 (2022). PubMed: 33526844
- Lee JJ et al. Galectin-3 Inhibitors Suppress Anoikis Resistance and Invasive Capacity in Thyroid Cancer Cells. Int J Endocrinol 2021:5583491 (2021). PubMed: 34035807
- Zhu D et al. Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling. Diabetol Metab Syndr 13:89 (2021). PubMed: 34446088
- Li Z & Chen B DUSP4 alleviates LPS-induced chondrocyte injury in knee osteoarthritis via the MAPK signaling pathway. Exp Ther Med 22:1401 (2021). PubMed: 34650647
- Liang Y et al. Biological evaluation of linalool on the function of blood vessels. Mol Med Rep 24:N/A (2021). PubMed: 34713293