重组Anti-JNK1 + JNK2 + JNK3抗体[EPR16797-211] - BSA and Azide free (ab225572)
![Flow Cytometry (Intracellular) - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)](https://www.abcam.cn/ps/products/225/ab225572/Images/ab225572-322025-anti-jnk1jnk2jnk3-antibody-epr16797-211-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16797-211] to JNK1 + JNK2 + JNK3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis
Related conjugates and formulations
概述
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产品名称
Anti-JNK1 + JNK2 + JNK3抗体[EPR16797-211] - BSA and Azide free
参阅全部 JNK1 + JNK2 + JNK3 一抗 -
描述
兔单克隆抗体[EPR16797-211] to JNK1 + JNK2 + JNK3 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis
预测可用于: Monkey
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HeLa cells. IP: Jurkat whole cell extract. Flow Cyt (intra): HeLa cells
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常规说明
ab225572 is the carrier-free version of ab179461.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16797-211 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225572于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 48 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 48 kDa). |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5599 Human
- Entrez Gene: 5601 Human
- Entrez Gene: 5602 Human
- Entrez Gene: 26414 Mouse
- Entrez Gene: 26419 Mouse
- Entrez Gene: 26420 Mouse
- Entrez Gene: 116554 Rat
- Entrez Gene: 25272 Rat
see all -
别名
- C Jun kinase 2 antibody
- c Jun N terminal kinase 1 antibody
- c Jun N terminal kinase 2 antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with purified ab179461 at 1/180 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179461).
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![Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)](https://www.abcam.cn/ps/products/225/ab225572/Images/ab225572-322024-anti-jnk1jnk2jnk3-antibody-epr16797-211-immunoprecipitation.jpg)
Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)JNK1+JNK2+JNK3 were immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract with ab179461 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179461 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Jurkat whole cell extract. Lane 2: PBS instead of Jurkat whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179461).
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![Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)](https://www.abcam.cn/ps/products/225/ab225572/Images/ab225572-286192-anti-jnk1jnk2jnk3-antibody-epr16797-211-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)This ICC/IF data was generated using the same anti-JNK1/2/3 antibody clone [EPR16797-211] in a different buffer formulation (cat# ab179461).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with ab179461 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: - ab179461 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. -
![Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)](https://www.abcam.cn/ps/products/225/ab225572/Images/ab225572-4-benefits-of-recombinant-antibodies.png)
Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (4)
ab225572 被引用在 4 文献中.
- Mahmoud NN et al. The Effect of Surface-Modified Gold Nanorods on the Early Stage of Embryonic Development and Angiogenesis: Insight into the Molecular Pathways. Int J Mol Sci 22:N/A (2021). PubMed: 34681694
- Li Y et al. Bilobalide protects against ischemia/reperfusion-induced oxidative stress and inflammatory responses via the MAPK/NF-?B pathways in rats. BMC Musculoskelet Disord 21:449 (2020). PubMed: 32646398
- Yang N et al. TNF-a receptor antagonist attenuates isoflurane-induced cognitive impairment in aged rats. Exp Ther Med 12:463-468 (2016). WB ; Rat . PubMed: 27347079
- Guo J et al. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evid Based Complement Alternat Med 2016:6983956 (2016). WB ; Human . PubMed: 27478481
